[Histonet] Intestinal mucus -- Carnoy or what?

[Tony Henwood (SCHN)]

Since the mucus will tend to be water soluble. I would suggest 10% formaldehyde in alcohol.
Fix for the usual time, dependant on size.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mikael Niku
Sent: Friday, 21 November 2014 9:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Intestinal mucus -- Carnoy or what?

Hello!

What is the best way to fix intestinal samples in order to preserve the mucus (and the embedded bacteria)?
I was recommended Carnoy's, but are there any alternatives... perhaps without chloroform?

With best regards,
Mikael

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