[Histonet] Intestinal mucus -- Carnoy or what? (and: dry methanol??)

[Mikael Niku]

Oh, sorry, I forgot one additional question regarding Carnoy's: in the 
protocols I found, Carnoy's is prepared using DRY methanol. Sorry for my 
ignorance, but does this mean that methanol has to be specifically 
treated to remove any hygroscopic etc water? Note that this is 
specifically intended for preserving intestinal mucus, which I guess 
might be sensitive to water-containing fixatives (although I presume 
there can't be more water in ordinary methanol than introduced by the 
tissue sample itself).

With best regards,
Mikael

On 21.11.2014 12:23, Mikael Niku wrote:
> Hello!
>
> What is the best way to fix intestinal samples in order to preserve 
> the mucus (and the embedded bacteria)?
> I was recommended Carnoy's, but are there any alternatives... perhaps 
> without chloroform?
>
> With best regards,
> Mikael
>
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