[Histonet] Jores, Klotz and that pesky chloral hydrate

[Tony Henwood (SCHN)]

Well if the specimens are well fixed in formalin, then bring back the colour with alcohol and you can the store them in a non-formalin solution such as dilute honey or high salt solution:

Henwood, A., (2002) "Color preservation in pathology museum specimens" Biotechnic & Histochem 77(4):230.

Özkan, N., Salva, E., Cakalagaoglu, F., & Tüzüner, B. (2012). Honey as a substitute for formalin?. Biotechnic & Histochemistry, 87(2), 148-153.

Al-Maaini R, Bryant P (2006) The effectiveness of honey as a substitute for formalin in the histological fixation of tissue. J. Histotechnol. 29: 173–176.

Oliveira, F. S. (2014). Assessing the effectiveness of 30% sodium chloride aqueous solution for the preservation of fixed anatomical specimens: a 5‐year follow‐up study. J. Anat. (2014) 225, pp118-121

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Molly Murphy
Sent: Friday, 21 November 2014 8:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate

Hi All,
I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle).

Does anyone have any thoughts about just leaving the chloral hydrate out?
Or, an alternative fixative that doesn't have the chloral hydrate?  I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two.

Thanks a bunch,
M

--
Molly Murphy DVM, Ph.D
Assistant Professor of Veterinary Pathology College of Natural Sciences & Mathematics University of Alaska Fairbanks
Office: (907) 474-1990
Fax: (907) 474-1932
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