[Histonet] Jores, Klotz and that pesky chloral hydrate

[Betty Pollock]

We are a veterinary pathology teaching lab and we used Klotz with chloral hydrate to preserve our gross teaching specimens.  We switched to a Klotz recipe that does not use chloral hydrate because the chloral hydrate is hard to source and very expensive.  It is maybe not quite as good as the Klotz with chloral hydrate but seems to work OK.

Here is the recipe.
Sodium chloride	208 g
Sodium bicarbonate	375 g
Sodium sulfate	458 g
Formaldehyde, 37%	667 ml
Water	To make up to 50 L 

Regards,

Betty Pollock
Manager, Operations DSU
 
Faculty of Veterinary Medicine
University of Calgary 
Calgary, AB, Canada
Tel: 403-220-2806
FAX: 403-239-6984


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Molly Murphy
Sent: Thursday, November 20, 2014 2:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate

Hi All,
I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle).

Does anyone have any thoughts about just leaving the chloral hydrate out?
Or, an alternative fixative that doesn't have the chloral hydrate?  I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two.

Thanks a bunch,
M

--
Molly Murphy DVM, Ph.D
Assistant Professor of Veterinary Pathology College of Natural Sciences & Mathematics University of Alaska Fairbanks
Office: (907) 474-1990
Fax: (907) 474-1932
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