[Histonet] TC nuclear stain

[Patsy Ruegg]

TC experts.  One of my former students is doing a TC stain and the nuclear stain with iron hematoxylin is washing out.  I remember that I had this problem myself with weigerts/iron heme, I always got a weaker purple stain rather than black like it was supposed to be.  Do you think it would help to repeat the nuclear stain at the end of the stain as well as in the beginning, make fresh hematoxylin, etc????

Thank you for your advise,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
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