[Histonet] RE:CSH 2014
Chung, Fai
Fai.Chung <@t> cshs.org
Mon Nov 3 12:50:12 CST 2014
I have not receive the certificates for the CSH back in May. I asked several time for the CE because I have many employees that needs it for reimbursement. In our institution, we only have 3 months to submit the documentation so all of us did not get the reimbursement and I don't know if anyone will be encourage to attend next time.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, November 03, 2014 10:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 132, Issue 3
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. FSH Newsletter (John Shelley)
2. bone paraffin embedding (Nicole Cosenza)
3. RE: bone paraffin embedding (Wineman, Terra)
4. Histology Technical Specialist - New England (Matt Ward)
5. RE: bone paraffin embedding (Jack Ratliff)
6. RE: bone paraffin embedding (Jack Ratliff)
7. anti-roll plate damage? (Bain,Virginia)
8. California Society for Histotechnology Meeting May 2014
(Maria Samaan)
----------------------------------------------------------------------
Message: 1
Date: Mon, 3 Nov 2014 15:22:01 +0000
From: John Shelley <jshelley <@t> sanfordburnham.org>
Subject: [Histonet] FSH Newsletter
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C54F513DA7DA7547B37103A4B74BDAE903CCA950 <@t> CARRERA.ln.burnham.org>
Content-Type: text/plain; charset="us-ascii"
Good Morning,
I wanted to share with everyone the Florida Society of Histotechnology's Fall newsletter. We have a great article on immunohistochemistry that will allow you to get 1 CEU credit if you submit your answers to our Continuing Education Chairperson. If you are a FSH member it is free and for non-members it will be a $10.00 charge. If you have the desire to submit an article please send your request to our Newsletter Editor and Chairperson. The names and emails to get in touch with the chairpersons are on page 2 of the newsletter. I have attached the link to get to the newsletter quicker, I hope you enjoy!!!
http://www.fshgroup.org/wp-content/uploads/2014/11/FSH-Newsletter-October-2014.pdf
Kind Regards!
John J Shelley
2014-2016 FSH President
------------------------------
Message: 2
Date: Mon, 03 Nov 2014 09:28:01 -0600
From: Nicole Cosenza <ncosenza <@t> siumed.edu>
Subject: [Histonet] bone paraffin embedding
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <54579F01.3070000 <@t> siumed.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Histonetters:
Our lab needs to paraffin embed and cut bone. Is there a special
process for fixation of bone, or can it be harvested and dropped right
into NBF?
--
Nicole Cosenza
Research Technician
Institute for Plastic Surgery
SIU School of Medicine
Springfield, Il
217.545.3862
------------------------------
Message: 3
Date: Mon, 3 Nov 2014 15:37:37 +0000
From: "Wineman, Terra" <Terra.Wineman <@t> novusint.com>
Subject: RE: [Histonet] bone paraffin embedding
To: Nicole Cosenza <ncosenza <@t> siumed.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1EB8F245A303564EADF12AC7022FA74DCBBC049B <@t> novus-ex01>
Content-Type: text/plain; charset="us-ascii"
Yes 1:20 dilution
Terra Wineman, HTL (ASCP)CM
Research Biologist, Nutritional Physiology
636-926-7476 phone
terra.wineman <@t> novusint.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nicole Cosenza
Sent: Monday, November 03, 2014 9:28 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] bone paraffin embedding
Histonetters:
Our lab needs to paraffin embed and cut bone. Is there a special process for fixation of bone, or can it be harvested and dropped right into NBF?
--
Nicole Cosenza
Research Technician
Institute for Plastic Surgery
SIU School of Medicine
Springfield, Il
217.545.3862
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Mon, 3 Nov 2014 10:56:40 -0500
From: "Matt Ward" <mw <@t> personifysearch.com>
Subject: [Histonet] Histology Technical Specialist - New England
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <064b01cff77e$c2f3a0c0$48dae240$@personifysearch.com>
Content-Type: text/plain; charset="us-ascii"
Good morning,
We are currently searching for a histology professional to join one of our
top clients. The position will be a field based support specialist and will
cover New England. The position offers a very competitive salary, bonus,
company car, gas card, cell phone, and laptop.
The company is requiring the candidate to have a 4 year degree and
experience in histology.
Please contact me directly to learn more - mw <@t> personifysearch.com
<mailto:mw <@t> personifysearch.com> .
Thanks!
Matt
Matt Ward
Program Manager
Personify
5020 Weston Parkway Suite 315
Cary NC 27513
(Tel) 919.459.3654
(Tel) 800.875.6188 direct ext 103
(Fax) 919.882.8727
<http://www.personifysearch.com/> www.personifysearch.com
------------------------------
Message: 5
Date: Mon, 3 Nov 2014 12:07:06 -0500
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: RE: [Histonet] bone paraffin embedding
To: Nicole Cosenza <ncosenza <@t> siumed.edu>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Cc: Jack Ratliff <ratliffjack <@t> gmail.com>,
"sarah_mack <@t> urmc.rochester.edu" <sarah_mack <@t> urmc.rochester.edu>,
"kim <@t> nsh.org" <kim <@t> nsh.org>
Message-ID: <COL128-W315054414A6367CFB6D8B6AE990 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Nicole,
You can very easily fix the bone in 10% NBF and then go into your decalcification process. Remember that fixation rate of bone is generally around 1mm per 24 hours (in all directions) and that it is good to have a minimum of 20:1 ratio of solutions to specimen size for each step. I would also recommend either 5% or 10% formic acid for decalcification.
Can you tell us more specifics about the bone and what you wish to accomplish histologically? This information would be
I might also suggest contacting Sarah Mack (copied to this message) from the University of Rochester. She is the new Hard Tissue Committee Chairperson for the National Society for Histotechnology and an expert in decalcified bone. I might also add that Sarah and Kim Simmons, NSH Education Development Manager, are working on educational opportunities and resource materials for those working with bone, biomaterials and medical device implants. If you are not a member of the NSH then you might want to consider becoming a member www.nsh.org/content/benefits-membership so that you can be kept up to date with what's coming soon from the Hard Tissue Committee.
Best Regards,
Jack
> Date: Mon, 3 Nov 2014 09:28:01 -0600
> From: ncosenza <@t> siumed.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] bone paraffin embedding
>
> Histonetters:
>
> Our lab needs to paraffin embed and cut bone. Is there a special
> process for fixation of bone, or can it be harvested and dropped right
> into NBF?
>
> --
> Nicole Cosenza
> Research Technician
> Institute for Plastic Surgery
> SIU School of Medicine
> Springfield, Il
> 217.545.3862
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Mon, 3 Nov 2014 12:29:13 -0500
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: RE: [Histonet] bone paraffin embedding
To: Nicole Cosenza <ncosenza <@t> siumed.edu>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Cc: Jack Ratliff <ratliffjack <@t> gmail.com>,
"sarah_mack <@t> urmc.rochester.edu" <sarah_mack <@t> urmc.rochester.edu>,
"kim <@t> nsh.org" <kim <@t> nsh.org>
Message-ID: <COL128-W796084284F2E30C9E96068AE990 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Sorry Nicole I forgot to finish a thought before I hit send. What I was going to say was any additional information you could provide regarding the specimen and what you wanted to see at the microscope can sometime yield additional information regarding specific processing protocols, staining, tips and tricks, etc.
Best,
Jack
From: ratliffjack <@t> hotmail.com
To: ncosenza <@t> siumed.edu; histonet <@t> lists.utsouthwestern.edu
CC: sarah_mack <@t> urmc.rochester.edu; kim <@t> nsh.org; ratliffjack <@t> gmail.com
Subject: RE: [Histonet] bone paraffin embedding
Date: Mon, 3 Nov 2014 12:07:06 -0500
Nicole,
You can very easily fix the bone in 10% NBF and then go into your decalcification process. Remember that fixation rate of bone is generally around 1mm per 24 hours (in all directions) and that it is good to have a minimum of 20:1 ratio of solutions to specimen size for each step. I would also recommend either 5% or 10% formic acid for decalcification.
Can you tell us more specifics about the bone and what you wish to accomplish histologically? This information would be
I might also suggest contacting Sarah Mack (copied to this message) from the University of Rochester. She is the new Hard Tissue Committee Chairperson for the National Society for Histotechnology and an expert in decalcified bone. I might also add that Sarah and Kim Simmons, NSH Education Development Manager, are working on educational opportunities and resource materials for those working with bone, biomaterials and medical device implants. If you are not a member of the NSH then you might want to consider becoming a member www.nsh.org/content/benefits-membership so that you can be kept up to date with what's coming soon from the Hard Tissue Committee.
Best Regards,
Jack
> Date: Mon, 3 Nov 2014 09:28:01 -0600
> From: ncosenza <@t> siumed.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] bone paraffin embedding
>
> Histonetters:
>
> Our lab needs to paraffin embed and cut bone. Is there a special
> process for fixation of bone, or can it be harvested and dropped right
> into NBF?
>
> --
> Nicole Cosenza
> Research Technician
> Institute for Plastic Surgery
> SIU School of Medicine
> Springfield, Il
> 217.545.3862
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Mon, 3 Nov 2014 17:34:06 +0000
From: "Bain,Virginia" <VEBain <@t> mdanderson.org>
Subject: [Histonet] anti-roll plate damage?
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <D07D14C0.8EF1%vebain <@t> mdanderson.org>
Content-Type: text/plain; charset="iso-8859-1"
Greetings,
Our lab recently got a new cryostat (a cryostar NX50). It sees light to
moderate usage (3 - 20 hours a week) and has been operational for about 3
weeks. It has had a host of problems since we received it (shipped with a
busted motor and the sensor in the window did not detect when it was shut
which lead to some kind of short and killed the lighting system). I am
hesitant to contact the rep again (they?ve told us they?re tired of
hearing from us) until I?m certain this is not user error of some sort.
For the most part we have been getting nice clean sections. Every so
often we start getting lines and tears which continue even when the blade
is moved or replaced. Twice now we have resolved this issue by rotating
the anti-roll plate to a smooth edge. When we encounter this problem I
find that the anti-roll plate is jagged to the touch, which is I think
what is causing the tears. I see that some people suggest smoothing the
anti-roll plate out on an emory board when it gets rough but this seems
very early in the life of the anti-roll plate to me. In my previous lab
we used the same anti-roll plate without issue for about 2 years with
heavy usage (50-70 hours a week) and so I?m wondering why this anti-roll
plate is encountering so much damage?
Thanks for the help!
--
Virginia Bain
Postdoctoral Fellow
Richie Lab
M D Anderson - Science Park
512-237-6443
------------------------------
Message: 8
Date: Mon, 3 Nov 2014 09:53:35 -0800
From: Maria Samaan <mmsamaan <@t> gmail.com>
Subject: [Histonet] California Society for Histotechnology Meeting May
2014
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAOjVhOsTL829dOWUFfhOwq0EA+9ePAb3nTM2me2ksNu-1dLf_w <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Greetings,
Has anyone received their credits for the California Society for
Histotechnology meeting which took place this May 2014, in Woodland Hills.
I know a few people who are still missing their credits and some need
paperwork for reimbursements.
Thanks,
Maria Samaan
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 132, Issue 3
****************************************
IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is strictly prohibited. Thank you for your cooperation.
More information about the Histonet
mailing list