[Histonet] Fat Stain on Paraffin Slide ?

[Bryan Llewellyn]

I always used Chiffele and Putt's oil red O in propylene glycol for 
paraffin 
sections(http://stainsfile.info/StainsFile/stain/lipid/chiffelleputtpropgly.htm).

Althugh triglycerides (i.e. "fat") is removed from tissues during 
paraffin processing, some lipids are not, or are only partially removed. 
Lipofuscins are an exaample, so is myelin to a lesser degree. usually 
the resistant lipids are those bound to protein. Oil red O is actually a 
good stain for lipofuscin. Of course, do not dehydrate or clear the 
section after staining, and use an aqueous mounting medium.

Bryan Llewellyn


Elizabeth Chlipala wrote:
> Forgive me if I'm missing something here but I'm under the impression that you can not stain for fat on paraffin section unless they have been rendered unsoluable via post fixation in something like osmium .  The fat would be removed during the processing cycle in the alcohols and xylenes.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Laboratory Manager
> Premier Laboratory, LLC
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
> Sent: Thursday, September 12, 2013 2:33 PM
> To: Stella Mireles; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Fat Stain on Paraffin Slide ?
>
> Sudan Black
> René J.
>
>
> ________________________________
> From: Stella Mireles <estellamireles <@t> gmail.com>
> To: "histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, September 12, 2013 1:15 PM
> Subject: [Histonet] Fat Stain on Paraffin Slide ?
>
>
> Ok Experts - Please advise me on a stain for Fat which I can do on paraffin sections?
> Could you expand on your own experience with such an animal.
>
> Thanks to All
> Stella
> Houston, Tx
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