[Histonet] Fat Stain on Paraffin Slide ?

[Elizabeth Chlipala]

Forgive me if I'm missing something here but I'm under the impression that you can not stain for fat on paraffin section unless they have been rendered unsoluable via post fixation in something like osmium .  The fat would be removed during the processing cycle in the alcohols and xylenes.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Laboratory Manager
Premier Laboratory, LLC
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, September 12, 2013 2:33 PM
To: Stella Mireles; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Fat Stain on Paraffin Slide ?

Sudan Black
René J.


________________________________
From: Stella Mireles <estellamireles <@t> gmail.com>
To: "histonet <@t> lists.utsouthwestern.edu" <Histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, September 12, 2013 1:15 PM
Subject: [Histonet] Fat Stain on Paraffin Slide ?


Ok Experts - Please advise me on a stain for Fat which I can do on paraffin sections?
Could you expand on your own experience with such an animal.

Thanks to All
Stella
Houston, Tx
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