[Histonet] (no subject)
Martin, Erin
Erin.Martin <@t> ucsf.edu
Mon Sep 9 08:28:29 CDT 2013
Good morning all!
One of our fellows emailed me a question that she came across while studying for her boards:
"I'm studying for my board exam and came across questions re: paraffin embedding.
It reads: best temperature for paraffin embedding is
38-48
48-58
58-70.
I am getting some info on Internet that says 58 but is the range lower or higher than that? What do we use?"
This seems to me to be an odd question because it depends on the melting point of the paraffin in use. Ours melts at 58C and we embed at 60C, but we have also used paraffin that melts at 56C and we embedded at 58C. Or am I missing something? Does anyone have a clear cut answer to this?
Thanks everyone!
Erin
Erin Martin, Histology Supervisor
UCSF Dermatopathology Service
415-353-7248
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