[Histonet] Cutting cells on PTFE membrane?

[Grantham, Andrea L - (algranth)]

I cut cells grown on membranes pretty often and just process them along with the other tissue. I usually put the membranes into a histoscreen cassette - don't wrap it or put anything on top of the membrane.
On embedding I usually cut the membrane into 2-3 sections and embed standing up to see the cells and how they attach to the membrane.
Works well - no problems.

Andi



Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu<mailto:algranth <@t> email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097