[Histonet] RE: Histonet Digest, Vol 115, Issue 11
Cesar Francisco Romero
chesarato <@t> hotmail.com
Tue Jun 11 13:48:56 CDT 2013
Her 2 Antibody
I do Manual IHC with Tissue Tek Slide Racks and have the same problem.
I think the problem is in the holder clip.
Only the top sample stains well.
Cesar Romero
Buenos Aires
Argentina
> From: histonet-request <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 115, Issue 11
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 11 Jun 2013 10:00:54 -0700
>
> Send Histonet mailing list submissions to
> histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. RE: samples (Webb, Dorothy L)
> 2. Re: Histonet Digest, Vol 115, Issue 10 (Wanda Shotsberger Gray)
> 3. RE: bone marrow aspirations (Cathy)
> 4. Her-2 antibody (Robert Fauck [CCDHB])
> 5. Heinz body stain on already dried blood smears.
> (Jackman, Mary Ione)
> 6. Telepathology (Gloria Tharp)
> 7. RE: Telepathology (Elizabeth Chlipala)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 10 Jun 2013 12:24:04 -0500
> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
> Subject: [Histonet] RE: samples
> To: "'sales <@t> royalmarker.com'" <sales <@t> royalmarker.com>
> Cc: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <65365F35C0F2EF4D846EC3CA73E49C4302758CF69446 <@t> HPEMX3.HealthPartners.int>
>
> Content-Type: text/plain; charset="utf-8"
>
> It is actually not a website but the internet communication (like a listserve) they have for PA’s. For instance, histology has one at histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu> that is used to communicate and network online.
>
> Thanks again!
>
> Dorothy Webb
>
> From: sales <@t> royalmarker.com [mailto:sales <@t> royalmarker.com]
> Sent: Monday, June 10, 2013 12:20 PM
> To: Webb, Dorothy L
> Subject: RE: samples
>
> No problem, the samples are on the way. How were you recommended for our product? Could you let us know which website you are referring to?
>
> Thanks Again,
> Shawn Jenkins
>
>
> -------- Original Message --------
> Subject: RE: samples
> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com<mailto:Dorothy.L.Webb <@t> HealthPartners.Com>>
> Date: Fri, June 07, 2013 1:40 pm
> To: "'sales <@t> royalmarker.com<mailto:sales <@t> royalmarker.com>'" <sales <@t> royalmarker.com<mailto:sales <@t> royalmarker.com>>
> Thank you very much. Your marking inks were recommended on the website for PA’s.
>
> Your efficient response is greatly appreciated and we will get back to you next week with feedback!
>
> Dorothy Webb
>
> From: sales <@t> royalmarker.com<mailto:sales <@t> royalmarker.com> [mailto:sales <@t> royalmarker.com]
> Sent: Friday, June 07, 2013 12:30 PM
> To: Webb, Dorothy L
> Subject: RE: samples
>
> Hello Dorothy,
>
> We will gladly send you a sample pack of our marking dyes to try. It will ship out today via UPS to the address below that you provided. I'll be sure to have your name as well as Jesse Spates on the label as well for a contact. The set will include 10 1/4oz bottles (all of our colors). Please contact us with any comments on the product. We appreciate your feedback and hope you are happy with our products. Have a great weekend and look forward to hearing from you.
>
> Best Regards,
> Shawn Jenkins
> Royal Marker
> (P) 954-462-0264
> (F) 954-374-7290
>
>
> -------- Original Message --------
> Subject: samples
> From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com<mailto:Dorothy.L.Webb <@t> HealthPartners.Com>>
> Date: Fri, June 07, 2013 11:59 am
> To: "'sales <@t> royalmarker.com<mailto:sales <@t> royalmarker.com>'" <sales <@t> royalmarker.com<mailto:sales <@t> royalmarker.com>>
> Cc: "Spates, Jesse D" <Jesse.D.Spates <@t> HealthPartners.Com<mailto:Jesse.D.Spates <@t> HealthPartners.Com>>
> Our PA’s are looking to try other inks than what we are currently using so we are wondering if we could please get some ink samples.
>
> Our hospital is Regions Hospital
> 640 Jackson St.
> St. Paul, MN. 55101
>
> I am the technical specialist of histology, Dorothy Webb and the lead PA is Jesse Spates @ 651-254-4913.
>
> Thank you,
>
> Dorothy Webb
> Regions Histology Technical Specialist
> 651-254-2962
>
>
> ________________________________
> This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
>
> If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0
>
> ------------------------------
>
> Message: 2
> Date: Mon, 10 Jun 2013 18:00:12 -0400
> From: Wanda Shotsberger Gray <wgray19 <@t> sc.rr.com>
> Subject: [Histonet] Re: Histonet Digest, Vol 115, Issue 10
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <qyl2qk6jorpq0tcykafkgfyo.1370901612237 <@t> email.android.com>
> Content-Type: text/plain; charset=utf-8
>
> Hi Megan,
> Welcome to the anatomic side! I just googled "history of tissue dyes" and a wealth of information popped up including this PDF: www.immunologie-labor.com/cellmarker_files/IET_reagents_07.pdf
> all about the discovery of hematoxylin and aniline dyes. The time period you mentioned was the time of these discoveries, so searching for history of hematoxylin or eosin should also get you good hits. Good luck!
> Wanda Shotsberger
> HT/HTL (ASCP)
>
> ------------------------------
>
> Message: 3
> Date: Mon, 10 Jun 2013 17:03:14 -0700
> From: "Cathy" <cfitz <@t> 007group.com>
> Subject: RE: [Histonet] bone marrow aspirations
> To: "'Shea's'" <jshea121 <@t> roadrunner.com>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <001b01ce6637$11eb70c0$35c25240$@007group.com>
> Content-Type: text/plain; charset="us-ascii"
>
> We are using a similar method at our site. The aspirate is placed into EDTA
> and then into formalin to fix. We pour the aspirate through a biopsy bag,
> open the bag and scrape the spicules together, transfer them to another
> biopsy bag and process as usual. I have received several compliments on the
> quality of the slides.
>
> Cathy
> Kelowna, B.C.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Shea's
> Sent: Saturday, June 1, 2013 4:26 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] bone marrow aspirations
>
> I am also interested in how others are processing bone marrow aspirates.
> Currently, we let the aspirate clot on a watch glass for about 20 minutes,
> gentle slide it onto filter paper, roll it around to get rid of the excess
> fluid, place the clot between sponges, and process as usual.
> However, I am interested in knowing if any one out there is using a
> technique that we use to use at another hospital 33 years ago.
> If I remember correctly, the pathologist or oncologist would collect the
> aspirate using a heparin syringe. Some how, the aspirate smears for
> Hematology were made on 22x22 cover slips instead of slides. Then the EDTA
> tube was then given to Histology. I can't remember if we added formalin or
> B5 fixative to it at that time, but it was filtered into an obex tea bag
> type filter paper (The blood never clotted and ran through the filter paper,
> leaving only bone marrow spicules in the paper). We would fold the paper,
> place it in the cassette, and process as usual. The pathologist preferred
> this method because he wouldn't have to look at levels of clot to find
> spicules. Is there anyone out there using this method?
> Jan
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 11 Jun 2013 17:04:35 +1200
> From: "Robert Fauck [CCDHB]" <Robert.Fauck <@t> ccdhb.org.nz>
> Subject: [Histonet] Her-2 antibody
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <9C92DFB6EA9983408AA9785182E9FC966804DE <@t> WN0NTEML11.AD.CCDHB.HEALTH.NZ>
> Content-Type: text/plain; charset=us-ascii
>
> Hi All
>
> We here in wellington Hospital New Zealand having some problem with the
> Her-2 ( Lieca, NCL-L-CBE-356 )
>
> Top half of the slide ( control or Test tissue) are strongly positive
> but if we put the control tissue on the bottom half it is week to
> negative!
>
> Does any one has the same problems with your Her-2, it is done on a
> Leica Bond (Max) III.
>
> Much appreciated for any suggestions, also from Leica Technical
> specialists.
>
> Regards,
> Robert Fauck
> Wellington hospital, NZ
>
>
> This email or attachment(s) may contain confidential or legally privileged information intended for the sole use of the addressee(s). Any use, redistribution, disclosure, or reproduction of this message, except as intended, is prohibited. If you received this email in error, please notify the sender and remove all copies of the message, including any attachments. Any views or opinions expressed in this email (unless otherwise stated) may not represent those of Capital & Coast District Health Board.
>
> http://www.ccdhb.org.nz
>
> (1C_S1)
>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 11 Jun 2013 13:34:09 +0000
> From: "Jackman, Mary Ione" <ione.jackman <@t> uconn.edu>
> Subject: [Histonet] Heinz body stain on already dried blood smears.
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <B01EDF48A11FDE4BADDB7E941AEE85E7195B1E8C <@t> MAILD.grove.ad.uconn.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Good Morning,
> Does anyone have a protocol they like for demonstrating Heinz bodies on already dried blood smears? There is no blood in tubes available any longer. I saw a citation for using Wrights Giemsa, but is there one that is more specific that anyone uses and is willing to share?
> Thanks.
> Ione Jackman
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 11 Jun 2013 09:08:40 -0500
> From: "Gloria Tharp" <gtharp <@t> pcasoutheast.com>
> Subject: [Histonet] Telepathology
> To: <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <006701ce66ad$2c971fc0$85c55f40$@com>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone have any recommendations for scanning equipment to have slides
> read off site from main lab. Thanks.
>
>
>
>
>
> Gloria Tharp, BA, HTL(ASCP)
>
> Director of Operations
>
> PCA Southeast
>
> gtharp <@t> pcasoutheast.com
>
> 931-490-1005
>
> 800-388-1294
>
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Tue, 11 Jun 2013 08:28:20 -0600
> From: Elizabeth Chlipala <liz <@t> premierlab.com>
> Subject: RE: [Histonet] Telepathology
> To: Gloria Tharp <gtharp <@t> pcasoutheast.com>,
> "Histonet <@t> lists.utsouthwestern.edu"
> <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <14E2C6176416974295479C64A11CB9AE016411AB4898 <@t> SBS2K8.premierlab.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Gloria
>
> There are many systems out there that perform scanning, your choice is dependent upon how you are going to be utilizing the instrument, how many slides per day that you will need to scan, if you will be utilizing any image analysis algorithms, etc.
>
> The DPA has lots information on its website - https://digitalpathologyassociation.org/
>
> They are also hosting a free webinar on July 10th (this is the 1st of 4) to sign up just go to this link https://digitalpathologyassociation.org/2013-dpa-webinar-series
> The webinar is titled Real-World Examples of Practical Consultative/Collaborative Digital Pathology Programs
> Wednesday, July 10 | 2 PM EST
>
> They also have a yearly conference, this year its in San Antonio you would be able to visit with many of the vendors that sell scanning systems - here is the link for that
> https://digitalpathologyassociation.org/pathology-visions-2013.
>
> Good Luck
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 881-0763 cell
> (303) 682-9060 fax
> liz <@t> premierlab.com
>
> Ship to address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gloria Tharp [gtharp <@t> pcasoutheast.com]
> Sent: Tuesday, June 11, 2013 8:08 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Telepathology
>
> Does anyone have any recommendations for scanning equipment to have slides
> read off site from main lab. Thanks.
>
>
>
>
>
> Gloria Tharp, BA, HTL(ASCP)
>
> Director of Operations
>
> PCA Southeast
>
> gtharp <@t> pcasoutheast.com
>
> 931-490-1005
>
> 800-388-1294
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 115, Issue 11
> *****************************************
More information about the Histonet
mailing list