[Histonet] RE: "Vacuole" appearance in lymph Nodes

[Britton, Josette C]

Try soaking the face of the block after facing on a wet ice-cube tray! 

Josie Britton HT/QIHC(ASCP)
Cheshire Medical Center
Keene, NH 03431

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony
Henwood (SCHN)
Sent: Thursday, February 14, 2013 6:35 PM
To: 'Metzger, Kenneth'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: "Vacuole" appearance in lymph Nodes

Ken,

Are the Microtomists being over enthusiastic with the block "trimming"
or "facing"?
This is more prone to happen if the block is cold (ie harder) during
trimming.
I would recommend after trimming to full-face that a few slower turns of
the microtome wheel at 4-5 microns will clear the holes (if this is
indeed the cause of the holes)

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Metzger,
Kenneth
Sent: Friday, 15 February 2013 3:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] "Vacuole" appearance in lymph Nodes

Hi All,

I have sent this question to respected colleagues individually so pardon
my redundancy if you have this email already.

Lately we have had random nodes show up with areas that appear to have
multiple vacuoles through-out the specimen.  They have different
patterns section to section so I know it is not in the tissue. The
staining looks fine and the tissue cuts well so I don't think it is a
processing issue. Any ideas on the problem would be welcome. Thanks

Ken


Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metzger <@t> aruplab.com


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