[Histonet] CD31 problewms

[Jo-Ann Bader, Ms.]

We are having problems getting our CD31 to work well on our NBF fixed mouse tissue. There is staining but it is very pale.

The CD31 comes from Bio-care and we are are using the IntelliPATH stainer.

We have tried:

- Trypsine 37C 15 min / Trypsine RT 30 min
- Trypsine (1:2 and 1:3)
- With Decloaker / without decloaker
- DAB Sparkle / No DAB sparkle
- Different dilution of CD31 (1:50 / 1:25)
- Different time of CD31 incubation (2h / 3h)
- Different time of incubation of secondary and tertiary reagent (10min to 20min)

Thanks


Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270