[Histonet] RE: IHC Technician job description

Davis, Cassie CDavis <@t> che-east.org
Mon Apr 22 09:56:22 CDT 2013


Vincent, you might want check out CLIA 88' complex testing requirement before you write that job description.

Cassandra Davis
CDavis <@t> che-east.org
302-575-8095
Saint Francis Hospital
Saintfrancishealthcare.org
Saint Francis Facebook Page
________________________________________
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Sent: Friday, April 19, 2013 1:08 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 113, Issue 20

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Today's Topics:

   1. Fwd: [Histonet] AutoTech Embedder( Sakura) (Kiranjit Grewal)
   2. New Lab In KY (Hale, Meredith)
   3. email (McKenzie, Emily)
   4. Calcium Stains? (McKenzie, Emily)
   5. RE: Calcium Stains? (Roberta Horner)
   6. Biosafety in the Mohs Laboratory (Zimmerman, Billie)
   7. looking for control block of "Burkitt Lymphoma" (Hammel, Vicky)
   8. IHC question (Shirley A. Powell)
   9. VCP-2001 cassette printer question about ink (Wayne D Gill)
  10. atlanta employment  (Michael Bourgeois)
  11. RE: RE: Pituitary gland (Tony Henwood (SCHN))
  12. RE: RE: Pituitary gland (Tony Henwood (SCHN))
  13. Re: Isopentane storage-the polling is open (Maria Mejia)
  14. RE: Calcium Stains? (Tony Henwood (SCHN))
  15. 2013 CAP Inspection Success & National Lab (Ian R Bernard)
  16. FNA preparation (Hannen, Valerie)
  17. IHC Technician job description (Vincent Rivera)


----------------------------------------------------------------------

Message: 1
Date: Thu, 18 Apr 2013 10:13:23 -0700
From: Kiranjit Grewal <kiran_g <@t> sbcglobal.net>
Subject: Fwd: [Histonet] AutoTech Embedder( Sakura)
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <D54D92EB-04D4-490E-B059-7504EC477758 <@t> sbcglobal.net>
Content-Type: text/plain;       charset=us-ascii

Hello,

I really need some feedback on this before we invest in this technology.

Thanks.

Kiran
Sent from my iPhone

Begin forwarded message:

> From: Kiranjit Grewal <kiran_g <@t> sbcglobal.net>
> Date: April 17, 2013, 2:25:36 PM PDT
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] AutoTech Embedder( Sakura)
>
> Hello Dear Colleagues,
>
> We are considering Sakura's AutoTech Embedder for embedding some of the tissues especially Large tissue. Any input or feedback is much appericiated.
>
> Anyone currently using it and would like to share their experience is great.
>
> Thank you,
>
> -Kiran
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Message: 2
Date: Thu, 18 Apr 2013 17:18:02 +0000
From: "Hale, Meredith" <mhale <@t> MiracaLS.com>
Subject: [Histonet] New Lab In KY
To: "Histonet <@t> lists.utsouthwestern.edu"
        <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <0E828EC51C7CC445A51E53F81B64E8C72242CB <@t> s-irv-exchmb.PathologyPartners.intranet>

Content-Type: text/plain; charset="us-ascii"

Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is  a multi-physician practice located in Northern Kentucky. Its mission is "To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems"  Looking for 2 Full Time HT/HTL's  ( 32 hours a week is full time employment at this practice )

*         Meet CLIA Grossing Requirements : CFR  493.1489,  http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens

*         Supervisor experience preferred

*         HT/HTL ASCP Certified

*         Experience with CLIA and CAP

*         Experience writing  and maintaining policies and procedures

*         Prior laboratory start up experience is preferred

*         Ability to work independently
Duties include:

*         Grossing

*         Embedding

*         Microtomy

*         Staining; routine and special stains only

*         Maintain supply orders and laboratory budget

*         Ability to be flexible and take on additional duties' as needed

*         Ability to work independently

*         Maintenance of laboratory for inspections

*         Maintenance of quality records






Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mhale <@t> miracals.com<mailto:mhale <@t> miracals.com>


Meredith Hale HT  (ASCP)cm
Director External Sales Support

Miraca Life Sciences
6655 North MacArthur Blvd.
Irving , Texas 75039
Office: 214-596-2219
Cell: 469-648-8253
Fax: 1-866-688-3280
mhale <@t> miracals.com<mailto:mhale <@t> miracals.com<mailto:mhale <@t> miracals.com%3cmailto:mhale <@t> miracals.com>>



------------------------------

Message: 3
Date: Thu, 18 Apr 2013 12:36:27 -0500
From: "McKenzie, Emily" <McKenzie.Emily <@t> mhsil.com>
Subject: [Histonet] email
To: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <FCDCEC5C9D3DEF479965D2EF67FEB2A439975E8C <@t> mmc-mail.ad.mhsil.com>
Content-Type: text/plain; charset="us-ascii"

Mckenzie.emily <@t> mshil.com



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------------------------------

Message: 4
Date: Thu, 18 Apr 2013 12:42:37 -0500
From: "McKenzie, Emily" <McKenzie.Emily <@t> mhsil.com>
Subject: [Histonet] Calcium Stains?
To: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <FCDCEC5C9D3DEF479965D2EF67FEB2A439975E8D <@t> mmc-mail.ad.mhsil.com>
Content-Type: text/plain; charset="us-ascii"

We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue?



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------------------------------

Message: 5
Date: Thu, 18 Apr 2013 14:15:15 -0400
From: Roberta Horner <rjr6 <@t> psu.edu>
Subject: [Histonet] RE: Calcium Stains?
To: "McKenzie, Emily" <McKenzie.Emily <@t> mhsil.com>,
        "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <F12BB68C2BA80B4686A8EA261F80DD45011471619855 <@t> pdc.padlspsu.psu.edu>
Content-Type: text/plain; charset="us-ascii"

I use Dahl's Calcium stain
Roberta Horner HT/HTL
Penn State University
Animal Diagnostic Lab

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of McKenzie, Emily
Sent: Thursday, April 18, 2013 1:43 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Calcium Stains?

We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue?



  ________________________________
This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
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------------------------------

Message: 6
Date: Thu, 18 Apr 2013 18:32:30 +0000
From: "Zimmerman, Billie" <BZIMMERM <@t> gru.edu>
Subject: [Histonet] Biosafety in the Mohs Laboratory
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <7B3DEB32E69C034EACB479059C5DE3FF7673A7 <@t> EX-MLB-03.ad.georgiahealth.edu>

Content-Type: text/plain; charset="us-ascii"

After being well acquainted with the CAP question, in regards to decontamination of cryostats, I would like some feedback as to how some of you perform this function.
The lower cost cryostats do not contain a fumigation cycle but instruct the user to use alcohol and/or some type of germicidal spray for decontamination.  I am hesitant about this and wondering if I'm just "old school" believing  in formaldehyde fumigation for eliminating Hepatitis, HIV, and TB. In the cryostat manual, there is no mention of fumigation and the cleaner they suggest is not sold in the United States.  Thanks in advance for your help!
Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to BZIMMERM <@t> gru.edu. Please update your address book to reflect this change.


------------------------------

Message: 7
Date: Thu, 18 Apr 2013 13:56:01 -0500
From: "Hammel, Vicky" <VHammel <@t> primecare.org>
Subject: [Histonet] looking for control block of "Burkitt Lymphoma"
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1E0E2B14C709174B8AC2BE0AE7F76833A2F2326ADB <@t> EXCHANGE2K7.staprimecare.org>

Content-Type: text/plain; charset="iso-8859-1"

I am looking for a tissue block that contains "Burkitt Lymphoma",  I have tissue with H.pylori, melanoma and amyloid that I would be willing to trade. Thank you,

Vicky Hammel HTL, ASCP
Pathology Technical Consultant

vhammel <@t> primecare.org<mailto:vhammel <@t> primecare.org>

St. Alexius Medical Center
Histology Laboratory
900 east Broadway
Bismarck, ND 58506

________________________________
This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message.



------------------------------

Message: 8
Date: Thu, 18 Apr 2013 15:42:58 -0400
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: [Histonet] IHC question
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <9BF995BC0E47744E9673A41486E24EE2579A9DDBFC <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="us-ascii"

I need to talk to any histotechs/researchers out there who are doing IHC on shellfish or fish.   Please contact me personally at powell_sa <@t> mercer.edu<mailto:powell_sa <@t> mercer.edu>.  Also any vendors who sell detection systems for them, please contact me.


Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA  31207
478-301-2374 Lab
478-301-5489 Fax



------------------------------

Message: 9
Date: Thu, 18 Apr 2013 16:32:11 -0400
From: Wayne D Gill <wdgill04 <@t> gmail.com>
Subject: [Histonet] VCP-2001 cassette printer question about ink
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <9765A008-719C-4B5A-955F-22D8013CC9BE <@t> gmail.com>
Content-Type: text/plain; charset=us-ascii

Does anyone know where to buy the ink ribbon for the VCP 2001 cassette printer?  Vendor and order # would be helpful.  Thank you.

  -Wayne


------------------------------

Message: 10
Date: Thu, 18 Apr 2013 17:11:50 -0400
From: "Michael Bourgeois" <mbourgeois <@t> finantempleton.com>
Subject: [Histonet] atlanta employment
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <006601ce3c79$58100f80$08302e80$@com>
Content-Type: text/plain;       charset="us-ascii"

Finan Templeton Dermatopathology Associates, is seeking an  experienced,
intelligent, positive, dynamic and certified
histotechnician/histotechnologist to become a member of our extraordinary
team. The ideal candidate functions well in a team environment, has
exceptional histological skills, and possesses ASCP certification.

We provide a full range of benefits for all full-time team members including
a generous profit sharing/retirement plan, paid vacation/sick time, and
medical, dental, and vision insurance plans.  Part-time team members receive
partial benefit packages based upon hours worked.  All Finan Templeton team
members receive generously competitive wages along with a commitment to an
environment of care, integrity, and mutual respect.



Michael Bourgeois

Lab Manager/Human Resources Manager

(404)851-1766 | Fax (404)851-1767 | mdb <@t> finantempleton.com

6095 Barfield Road Suite 200 | Atlanta, GA 30342









------------------------------

Message: 11
Date: Thu, 18 Apr 2013 22:54:19 +0000
From: "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>
Subject: RE: [Histonet] RE: Pituitary gland
To: "'Geoff'" <mcauliff <@t> umdnj.edu>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <6D6BD1DE8A5571489398B392A38A71579D2420E7 <@t> xmdb04.nch.kids>
Content-Type: text/plain; charset="us-ascii"

Oh I am getting too old (and I was trying to be modern!)

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Geoff
Sent: Friday, 19 April 2013 12:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Pituitary gland

In our library all of these are still known as /Stain Technology/. The name change to /Biotechnic and Histochemistry/ happened much later.

Geoff


On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote:
> Some references that might be useful:
>
> Paget, G. E., & Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic & Histochemistry, 35(3), 119-122.
>
> Phifer, R. F., Spicer, S. S., & Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569.
>
> Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic & Histochemistry, 34(6), 343-346.
>
>
> Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80.
>
>
>
> Read More:
> http://informahealthcare.com/doi/abs/10.3109/10520295909114648
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001,
> Westmead NSW 2145, AUSTRALIA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Bustamante, Lin
> Sent: Thursday, 18 April 2013 1:45 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Pituitary gland
>
> Looking for any stain that shows acidophilic and basophilic cell from pituitary gland.
> Thank you.
> Lin.
>
>
> Lin S. Bustamante, B.S., H.T.(ASCP)
> VIBS Histology Laboratory Supervisor
> College Of Veterinary Medicine
> Texas A&M University
> College Station, Texas 77843-4458
> Phone: (979) 845-3177
> Fax: (979) 458-3499
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> **********************************************************************
> *********** This email and any files transmitted with it are
> confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.
>
> Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network.
>
> This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses.
> **********************************************************************
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>
> _______________________________________________
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--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
**********************************************

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Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network.

This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses.
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------------------------------

Message: 12
Date: Thu, 18 Apr 2013 23:25:52 +0000
From: "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>
Subject: RE: [Histonet] RE: Pituitary gland
To: "'Houston, Ronald'" <Ronald.Houston <@t> nationwidechildrens.org>,
        Geoff   <mcauliff <@t> umdnj.edu>, "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <6D6BD1DE8A5571489398B392A38A71579D2420FD <@t> xmdb04.nch.kids>
Content-Type: text/plain; charset="us-ascii"

Yep,

I missed one reference (by Prof Pearse) which I agree is my preferred technique:

Everson Pearse, A. G. (1950). Differential stain for the human and animal anterior hypophysis. Biotechnic & Histochemistry, 25(2), 95-102.

And where I used the stain:     Henwood, A.F., (1983) "Crossreactivity in Immunoperoxidase: Human Placental Lactogen and the Pituitary".  N.Z. J. Med.Lab Tech.  37(2):29-31.

I am proud to remember that I met Professor Pearse (God rest his soul) many years ago at an IAP meeting in Sydney - An icon (like Lee Luna, Russ Allison and Chuck Churukian)

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Houston, Ronald
Sent: Friday, 19 April 2013 12:48 AM
To: Geoff; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Pituitary gland

back in the days when I worked exclusively in neuropathology in Glasgow, the tinctorial stains of choice for pituitary exams were PAS/OG and OFG (orange fuchsin green) methods.  I don't have the references to hand , although I believe the first was attributed to Tony Pearse, but can probably pull up the methods if needed


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston <@t> nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Geoff
Sent: Thursday, April 18, 2013 10:29 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Pituitary gland

In our library all of these are still known as /Stain Technology/. The name change to /Biotechnic and Histochemistry/ happened much later.

Geoff


On 4/17/2013 9:14 PM, Tony Henwood (SCHN) wrote:
> Some references that might be useful:
>
> Paget, G. E., & Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic & Histochemistry, 35(3), 119-122.
>
> Phifer, R. F., Spicer, S. S., & Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569.
>
> Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic & Histochemistry, 34(6), 343-346.
>
>
> Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic & Histochemistry, 34(2), 77-80.
>
>
>
> Read More:
> http://informahealthcare.com/doi/abs/10.3109/10520295909114648
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Laboratory Manager & Senior Scientist
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001,
> Westmead NSW 2145, AUSTRALIA
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
> Bustamante, Lin
> Sent: Thursday, 18 April 2013 1:45 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Pituitary gland
>
> Looking for any stain that shows acidophilic and basophilic cell from pituitary gland.
> Thank you.
> Lin.
>
>
> Lin S. Bustamante, B.S., H.T.(ASCP)
> VIBS Histology Laboratory Supervisor
> College Of Veterinary Medicine
> Texas A&M University
> College Station, Texas 77843-4458
> Phone: (979) 845-3177
> Fax: (979) 458-3499
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> **********************************************************************
> *********** This email and any files transmitted with it are
> confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.
>
> Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network.
>
> This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses.
> **********************************************************************
> ***********
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
**********************************************

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network.

This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses.
*********************************************************************************



------------------------------

Message: 13
Date: Thu, 18 Apr 2013 17:10:47 -0700
From: Maria Mejia <mbmphoto <@t> gmail.com>
Subject: Re: [Histonet] Isopentane storage-the polling is open
To: mwhite <@t> mcleodhealth.org
Cc: HistoNet <histonet <@t> lists.utsouthwestern.edu>,
        histonet-bounces <@t> lists.utsouthwestern.edu, "Morken,     Timothy"
        <Timothy.Morken <@t> ucsfmedctr.org>
Message-ID: <5D1B41E9-9905-4EAB-A5F0-52E9C69113E8 <@t> gmail.com>
Content-Type: text/plain; charset=us-ascii

Melanie,

Who's your vendor for buying Novec 7000?

Maria Mejia
San Francisco, CA
Affymetrix
On Apr 17, 2013, at 10:08 AM, mwhite <@t> mcleodhealth.org wrote:

> I just wanted to clarify that we don't use Novec 7000 for freezing of
> muscle tissue. We use it for other routine frozen sections on
> humans.....Sorry if that caused any confusion. Thanks Tim for pointing that
> out!
>
>
>
>
> Melanie S. White, MT(ASCP)
> Laboratory Supervisor, Systems/Anatomic Pathology
> McLeod Regional Medical Center
> (843) 777-2072
>
>
>
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------------------------------

Message: 14
Date: Fri, 19 Apr 2013 00:16:22 +0000
From: "Tony Henwood (SCHN)" <tony.henwood <@t> health.nsw.gov.au>
Subject: [Histonet] RE: Calcium Stains?
To: "'McKenzie, Emily'" <McKenzie.Emily <@t> mhsil.com>,
        "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <6D6BD1DE8A5571489398B392A38A71579D24213D <@t> xmdb04.nch.kids>
Content-Type: text/plain; charset="us-ascii"

Emily,

I would suggest the alizarin Red S Technique.
Since silver substitutes calcium in the Von Kossa's technique, it can therefore be regarded as a histochemical test for inorganic phosphates rather than calcium (see Meloan & Puchtler (1985) J Histotechnol 8(1):11-13).

Alizarin Red S for Calcium allows the direct demonstration of insoluble calcium cations where Alizarin Red S forms an insoluble orange red lake.  The technique is made specific by pH control (Garvey et al 1989, J Histotechnol 12 (3):225-227). Alizarin Red S also forms lakes with other metals such as barium, aluminium, mercury and magnesium. Fortunately, the bright red colouration of calcium can be easily differentiated from the dark red of other metal-lakes.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of McKenzie, Emily
Sent: Friday, 19 April 2013 3:43 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Calcium Stains?

We are having some trouble with our Von Kossa Calcium stain. Are there other stains that could be suggested to stain for Calcium in patient tissue?



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------------------------------

Message: 15
Date: Fri, 19 Apr 2013 10:31:26 +0000
From: Ian R Bernard <ibernard <@t> uab.edu>
Subject: [Histonet] 2013 CAP Inspection Success & National Lab
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Cc: "BERNARD, IAN R MSgt USAF USAFA 10 MDSS/SGSH"
        <ian.bernard <@t> us.af.mil>
Message-ID:
        <D4F4C602B10B9F45B4E9271AF6380E16181B65C1 <@t> UABEXMB1.ad.uab.edu>
Content-Type: text/plain; charset="us-ascii"

Colleagues, thank you for your input with questions.  Your input and our implementation have proved successful.

Hard work towards alignment of policies, procedures and practices to standards (CAP) all proved successful.  Thank you to my staff, in particular SRA George Williams, for their hard work in preparation.

Yesterday, 18th April 13, we were inspected by CAP and we are glad to report that we had a very successful outcome: Our Anatomic Pathology department scored with: "Zero Discrepancies and Recommendations".

A very detailed inspection, in all aspects of our entire laboratory with 10 inspectors.  Bottom-line: Our Lab is reaccredited another two years to serve our patient population.

Also glad to report that prior to the inspection that our lab was selected as the 2013 Advance Magazine National Lab of the Year! Please see the April edition of Advance Magazine.

Fellow Colorado Histo colleagues, I'll see you tomorrow for the Colorado Society of Histotechnology Meeting in Fort Collins.

V/r
Ian R. Bernard
Ian R. Bernard, MSHA, HT (ASCP)- (Working on HTL)
NCOIC-Manager, Anatomic Pathology Lab
10th Medical Group
USAF Academy, CO 80840
Graduate Certificate In Gerontology Student-UAB
210-687-7540



------------------------------

Message: 16
Date: Fri, 19 Apr 2013 10:43:05 -0400
From: "Hannen, Valerie" <Valerie.Hannen <@t> parrishmed.com>
Subject: [Histonet] FNA preparation
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <450B7A81EDA0C54E97C53D60F00776C3231B3D7C2A <@t> isexstore03>
Content-Type: text/plain; charset="us-ascii"

Good Morning fellow Histonetters!!

I am hoping someone can answer this question for me.  Is a General Lab Tech (working as an assistant) permitted to prepare FNA slides at the bedside, as well as

stain them using the Wright Stain before the slides being to the Pathologist for interpretation?

 My thinking on it is that I believe that they should be able to because after all they make CBC slides and stain those.

 We are having a debate in our department and I would like to know what the correct answer is before I would implement any training for the General tech.

Thanks ever so much!!

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com


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------------------------------

Message: 17
Date: Fri, 19 Apr 2013 16:54:37 +0000
From: Vincent Rivera <vrivera <@t> westderm.com>
Subject: [Histonet] IHC Technician job description
To: "'histonet <@t> lists.utsouthwestern.edu'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <3D4A471B82E7A44C87F6839732320D9F017C72 <@t> VSPDMS-ITEXMB02.DMS.COM>
Content-Type: text/plain; charset="us-ascii"

Hello fellow Histonetters:

Does anyone have an IHC technician / specialist job description that they would wish to share with me? I am in the process of creating a new "IHC tech" specific position, so any help is much appreciated.

Thank you,

Vincent Rivera, HT (ASCP) QIHC
Histopathology Supervisor
West Dermatology Pathology Laboratory
vrivera <@t> westderm.com<mailto:vrivera <@t> westderm.com>
714-924-7240 (Lab)
714-390-0906 (Cell)



------------------------------

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