[Histonet] Isopentane storage-the polling is open

[Bernice Frederick]

Always in an explosion proof refrigerator for storage. The isopentane in use is in an isopentane tank (at -70C) in the frozen section  area of the gross room as is the fridge....

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Garrison, Becky
Sent: Tuesday, April 16, 2013 2:17 PM
To: 'Paula Sicurello'; HistoNet
Subject: RE: [Histonet] Isopentane storage-the polling is open

We store at room temperature in a flammable cabinet.
Use it to freeze muscle biopsies.  The Isopentane is pre-cooled by immersing a container of isopentane in a container of Liquid Nitrogen.


Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237, phone
904-244-4290, fax
904-393-3194, pager
 
  
 
 
 
 
 
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, April 16, 2013 1:35 PM
To: HistoNet
Subject: [Histonet] Isopentane storage-the polling is open

Hello HistoNetters,

I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.)

Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature.

What do you all do?  Room temp or refrigerated?

Any and all comments are greatly appreciated.

Thanks!

Paula

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710
P:  919.684.2091
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