[Histonet] Isopentane storage-the polling is open

[Garrison, Becky]

We store at room temperature in a flammable cabinet.
Use it to freeze muscle biopsies.  The Isopentane is pre-cooled by immersing
a container of isopentane in a container of Liquid Nitrogen.


Becky Garrison
Pathology Supervisor
Shands Jacksonville
Jacksonville, FL 32209
904-244-6237, phone
904-244-4290, fax
904-393-3194, pager
 
  
 
 
 
 
 
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, April 16, 2013 1:35 PM
To: HistoNet
Subject: [Histonet] Isopentane storage-the polling is open

Hello HistoNetters,

I have been having a discussion with my boss about whether or not to store
the isopentane (used for freezing muscle biopsies) in the refrigerator. (An
expensive, explosion proof one that I would have to buy.)

Her only experience is with the isopentane stored in the refrigerator, my
experiences are with storing it at room temperature.

What do you all do?  Room temp or refrigerated?

Any and all comments are greatly appreciated.

Thanks!

Paula

-- 
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091
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