[Histonet] antibody diluent

[Rene J Buesa]

Margaret:
Prepare your own Ab diluent, as I did for many many years. Use the follwoing recipe:
 
Bovine serum albumen → 1 mL
PBS ---------------------------- 99 mL
Tween 20 -------------------- 50 µL
Sodium azide --------------- 100 µg
 
Albumen is a large molecule needed to prevent the Ab globulin molecules to sink keeping them in a homogeneous suspension.
Tween you know is the surfactant helping the Ab to disperse over the sections and Sodium azide is the preservative for the diluent. If you read the label on the Ab diluent you are using now, you will find the sodium azide at the same proportion in this recipe (0.01%).
Keep the diluent in the refrigerator (about 4ºC) and it will last for months
René J.

From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Tuesday, April 9, 2013 2:14 PM
Subject: [Histonet] antibody diluent

I am looking for a less expensive antibody diluent and would like some opinions on which companies are best .  Are there differences between diluents when it comes to background staining?

Margaret Perry HT(ASCP)
Veterinary & Biomedical Sciences Department
North Campus Drive Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638

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