[Histonet] Re: frozen slides
naveeda arshad
naveedafahim <@t> yahoo.ca
Mon Jun 18 20:08:59 CDT 2012
yes you can use charged slides for frozen section they work best In our lab we always use these slides we never had problemnaveeda
--- On Mon, 6/18/12, histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu> wrote:
From: histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu>
Subject: Histonet Digest, Vol 103, Issue 22
To: histonet <@t> lists.utsouthwestern.edu
Received: Monday, June 18, 2012, 9:07 PM
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Today's Topics:
1. Re: Frozen Sections Falling Off Slides (Emily Sours)
2. Re: Frozen Sections Falling Off Slides (Badz Yahoo)
3. Employee productivity (Scott, Allison D)
4. Re: Employee productivity (Rene J Buesa)
5. Fungus , GMS Aspergillus (Cheri Miller)
----------------------------------------------------------------------
Message: 1
Date: Sun, 17 Jun 2012 15:34:16 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] Frozen Sections Falling Off Slides
To: histonet <@t> lists.utsouthwestern.edu
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Wow you can run frozen sections through those machines? That's awesome. I
figured it wouldn't be gentle enough to process them.
I'm a fan of Fisher Scientific superfrost plus slides. Fisher claims the
slides are electrostatically charged to keep the sections from falling
off. Whether that's a bunch of hooha or it's actually true, our sections
don't fall off, even after in situs. I believe most scientific suppliers
sell their own brand of superfrost plus slides if you don't want to buy
from Fisher.
Emily
"You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir."
--Howard Moon, in "Charlie", The Mighty Boosh
On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio <one_angel_secret <@t> yahoo.com>wrote:
> Was your leica set that cold? Maybe you could try warming the slide for a
> couple seconds before putting in alcohol. As far a charged slides I am
> amazed with the slides Dako sells for their IHC.
>
> Sent from my iPhone
>
> On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario <badzrosari <@t> yahoo.com>
> wrote:
>
> > Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen
> section Machine...But i dont understand why i got problems sections falling
> off slides.I used different types of slides non adhesive,adhesive(poly l
> lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken
> from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative
> etc..then after distilled water wash out...sections started to peel
> off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of
> tissue- fibroma and salivary gland...
> > I used Leica cryostat previously but i never got these problems...Even i
> used non adhesive but sections still on the slides..Can someone advised
> please.Maybe youre using the same tissue tek cryo 3...
> > _______________________________________________
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> > Histonet <@t> lists.utsouthwestern.edu
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------------------------------
Message: 2
Date: Mon, 18 Jun 2012 16:38:57 +0300
From: Badz Yahoo <badzrosari <@t> yahoo.com>
Subject: Re: [Histonet] Frozen Sections Falling Off Slides
To: Emily Sours <talulahgosh <@t> gmail.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E54FA38A-C04E-4D22-8418-FBD7717FDF4C <@t> yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hello netters...have someone tried doin frozen in a tissue mistakenly immersed in formalin??not so long but the inside still fresh..m sure the slides will wash out..fresh always better..
On Jun 17, 2012, at 10:34 PM, Emily Sours <talulahgosh <@t> gmail.com> wrote:
> Wow you can run frozen sections through those machines? That's awesome. I
> figured it wouldn't be gentle enough to process them.
> I'm a fan of Fisher Scientific superfrost plus slides. Fisher claims the
> slides are electrostatically charged to keep the sections from falling
> off. Whether that's a bunch of hooha or it's actually true, our sections
> don't fall off, even after in situs. I believe most scientific suppliers
> sell their own brand of superfrost plus slides if you don't want to buy
> from Fisher.
>
> Emily
>
> "You see a peanut, day's off to a good start; you witness some soil it's a
> jamboree for Vince Noir."
> --Howard Moon, in "Charlie", The Mighty Boosh
>
>
>
> On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio <one_angel_secret <@t> yahoo.com>wrote:
>
>> Was your leica set that cold? Maybe you could try warming the slide for a
>> couple seconds before putting in alcohol. As far a charged slides I am
>> amazed with the slides Dako sells for their IHC.
>>
>> Sent from my iPhone
>>
>> On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario <badzrosari <@t> yahoo.com>
>> wrote:
>>
>>> Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen
>> section Machine...But i dont understand why i got problems sections falling
>> off slides.I used different types of slides non adhesive,adhesive(poly l
>> lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken
>> from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative
>> etc..then after distilled water wash out...sections started to peel
>> off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of
>> tissue- fibroma and salivary gland...
>>> I used Leica cryostat previously but i never got these problems...Even i
>> used non adhesive but sections still on the slides..Can someone advised
>> please.Maybe youre using the same tissue tek cryo 3...
>>> _______________________________________________
>>> Histonet mailing list
>>> Histonet <@t> lists.utsouthwestern.edu
>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Mon, 18 Jun 2012 15:55:09 +0000
From: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu>
Subject: [Histonet] Employee productivity
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<FA607DC3D1ED7C46A9546820A3EB877F20014B <@t> LBMSG02.hchd.local>
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Hello to all in histoland. How is employee productivity calculated. I have my techs fill out a workload tally sheet daily. My boss wants me to take the data and come up with a productivity chart or graph that says who is productive or not. I don't need a tally sheet to say who is working or not. Is any one doing this? I need help.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
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Message: 4
Date: Mon, 18 Jun 2012 09:01:37 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Employee productivity
To: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<1340035297.45993.YahooMailNeo <@t> web121406.mail.ne1.yahoo.com>
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With the data you have from your employees, calculate the number of units per unit time you get for the different tasks.
Feed your data on Excel and prepare totals per lab and per employee and make graphs that you can display and discuss with your boss and your employees.
René J.
________________________________
From: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, June 18, 2012 11:55 AM
Subject: [Histonet] Employee productivity
Hello to all in histoland. How is employee productivity calculated. I have my techs fill out a workload tally sheet daily. My boss wants me to take the data and come up with a productivity chart or graph that says who is productive or not. I don't need a tally sheet to say who is working or not. Is any one doing this? I need help.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
Houston, Texas 77026
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If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from
your computer system.
To the extent the information in this e-mail and any attachments contain
protected health information as defined by the Health Insurance Portability
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or
privileged. This e-mail may also be confidential and/or privileged under
Texas law. The e-mail is for the use of only the individual or entity named
above. If you are not the intended recipient, or any authorized
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Message: 5
Date: Mon, 18 Jun 2012 11:02:50 -0500
From: Cheri Miller <cmiller <@t> physlab.com>
Subject: [Histonet] Fungus , GMS Aspergillus
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Cc: "histonet-bounces <@t> lists.utsouthwestern.edu"
<histonet-bounces <@t> lists.utsouthwestern.edu>
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I am looking for natural occurring Fungus, GMS Aspergillus to use as my fungus control tissue. Someone from Histonet gave me a block about 3 years ago. I've just used the last of it. Does anyone have any to share again? Thanks Cheri
Cheryl A. Miller HT(ASCP)cm
Histology Supervisor
Physicians Laboratory Services
4840 F Street
Omaha, NE. 68127-0999
402 731 4145 ext. 554
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