[Histonet] 1. Re: Frozen Sections Falling Off Slides

Mon Jun 18 18:48:25 CDT 2012

Bernadette,
Tray to transfer frozen tissue to 50% formalin and 50%alcohol solution.

On Monday, June 18, 2012, wrote:

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> Today's Topics:
>
>   1. Re: Frozen Sections Falling Off Slides (Emily Sours)
>   2. Re: Frozen Sections Falling Off Slides (Badz Yahoo)
>   3. Employee productivity (Scott, Allison D)
>   4. Re: Employee productivity (Rene J Buesa)
>   5. Fungus , GMS Aspergillus  (Cheri Miller)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Sun, 17 Jun 2012 15:34:16 -0400
> From: Emily Sours <talulahgosh <@t> gmail.com <javascript:;>>
> Subject: Re: [Histonet] Frozen Sections Falling Off Slides
> To: histonet <@t> lists.utsouthwestern.edu <javascript:;>
> Message-ID:
>        <CAP=XX1y2cVUQ_qsNbguP=4Y4QEVnX+Jb0DRPENxPzsyokJfyNw <@t> mail.gmail.com<javascript:;>
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>
> Wow you can run frozen sections through those machines? That's awesome.  I
> figured it wouldn't be gentle enough to process them.
> I'm a fan of Fisher Scientific superfrost plus slides.  Fisher claims the
> slides are electrostatically charged to keep the sections from falling
> off.  Whether that's a bunch of hooha or it's actually true, our sections
> don't fall off, even after in situs.  I believe most scientific suppliers
> sell their own brand of superfrost plus slides if you don't want to buy
> from Fisher.
>
> Emily
>
> "You see a peanut, day's off to a good start; you witness some soil it's a
> jamboree for Vince Noir."
> --Howard Moon, in "Charlie", The Mighty Boosh
>
>
>
> On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio <one_angel_secret <@t> yahoo.com<javascript:;>
> >wrote:
>
> > Was your leica set that cold? Maybe you could try warming the slide for a
> > couple seconds before putting in alcohol. As far a charged slides I am
> > amazed with the slides Dako sells for their IHC.
> >
> > Sent from my iPhone
> >
> > On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario <
> badzrosari <@t> yahoo.com <javascript:;>>
> > wrote:
> >
> > > Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen
> > section Machine...But i dont understand why i got problems sections
> falling
> > off slides.I used different types of slides non adhesive,adhesive(poly l
> > lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections
> taken
> > from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative
> > etc..then after distilled water wash out...sections started to peel
> > off...My machine setting- chamber is -30 C;cryochamber is -39 C..example
> of
> > tissue- fibroma and salivary gland...
> > > I used Leica cryostat previously but i never got these problems...Even
> i
> > used non adhesive but sections still on the slides..Can someone advised
> >  please.Maybe youre using the same tissue tek cryo 3...
> > > _______________________________________________
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>
> ------------------------------
>
> Message: 2
> Date: Mon, 18 Jun 2012 16:38:57 +0300
> From: Badz Yahoo <badzrosari <@t> yahoo.com <javascript:;>>
> Subject: Re: [Histonet] Frozen Sections Falling Off Slides
> To: Emily Sours <talulahgosh <@t> gmail.com <javascript:;>>
> Cc: "histonet <@t> lists.utsouthwestern.edu <javascript:;>"
>        <histonet <@t> lists.utsouthwestern.edu <javascript:;>>
> Message-ID: <E54FA38A-C04E-4D22-8418-FBD7717FDF4C <@t> yahoo.com <javascript:;>
> >
> Content-Type: text/plain;       charset=us-ascii
>
> Hello netters...have someone tried doin frozen in a tissue mistakenly
> immersed in formalin??not so long but the inside still fresh..m sure the
> slides will wash out..fresh always better..
>
> On Jun 17, 2012, at 10:34 PM, Emily Sours <talulahgosh <@t> gmail.com<javascript:;>>
> wrote:
>
> > Wow you can run frozen sections through those machines? That's awesome.
>  I
> > figured it wouldn't be gentle enough to process them.
> > I'm a fan of Fisher Scientific superfrost plus slides.  Fisher claims the
> > slides are electrostatically charged to keep the sections from falling
> > off.  Whether that's a bunch of hooha or it's actually true, our sections
> > don't fall off, even after in situs.  I believe most scientific suppliers
> > sell their own brand of superfrost plus slides if you don't want to buy
> > from Fisher.
> >
> > Emily
> >
> > "You see a peanut, day's off to a good start; you witness some soil it's
> a
> > jamboree for Vince Noir."
> > --Howard Moon, in "Charlie", The Mighty Boosh
> >
> >
> >
> > On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio <
> one_angel_secret <@t> yahoo.com <javascript:;>>wrote:
> >
> >> Was your leica set that cold? Maybe you could try warming the slide for
> a
> >> couple seconds before putting in alcohol. As far a charged slides I am
> >> amazed with the slides Dako sells for their IHC.
> >>
> >> Sent from my iPhone
> >>
> >> On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario <
> badzrosari <@t> yahoo.com <javascript:;>>
> >> wrote:
> >>
> >>> Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen
> >> section Machine...But i dont understand why i got problems sections
> falling
> >> off slides.I used different types of slides non adhesive,adhesive(poly l
> >> lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections
> taken
> >> from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative
> >> etc..then after distilled water wash out...sections started to peel
> >> off...My machine setting- chamber is -30 C;cryochamber is -39
> C..example of
> >> tissue- fibroma and salivary gland...
> >>> I used Leica cryostat previously but i never got these problems...Even
> i
> >> used non adhesive but sections still on the slides..Can someone advised
> >> please.Maybe youre using the same tissue tek cryo 3...
> >>> _______________________________________________
> >>> Histonet mailing list
> >>> Histonet <@t> lists.utsouthwestern.edu <javascript:;>
> >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >>
> >> _______________________________________________
> >> Histonet mailing list
> >> Histonet <@t> lists.utsouthwestern.edu <javascript:;>
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >>
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu <javascript:;>
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 18 Jun 2012 15:55:09 +0000
> From: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu <javascript:;>>
> Subject: [Histonet] Employee productivity
> To: "histonet <@t> lists.utsouthwestern.edu <javascript:;>"
>        <histonet <@t> lists.utsouthwestern.edu <javascript:;>>
> Message-ID:
>        <FA607DC3D1ED7C46A9546820A3EB877F20014B <@t> LBMSG02.hchd.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello to all in histoland.  How is employee productivity calculated.  I
> have my techs fill out a workload tally sheet daily.  My boss wants me to
> take the data and come up with a productivity chart or graph that says who
> is productive or not.  I don't need a tally sheet to say who is working or
> not.  Is any one doing this?  I need help.
>
>
> Allison Scott HT(ASCP)
> Histology Supervisor
> LBJ Hospital
> Houston, Texas 77026
>
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> ------------------------------
>
> Message: 4
> Date: Mon, 18 Jun 2012 09:01:37 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com <javascript:;>>
> Subject: Re: [Histonet] Employee productivity
> To: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu <javascript:;>>,
>        "histonet <@t> lists.utsouthwestern.edu <javascript:;>"
>        <histonet <@t> lists.utsouthwestern.edu <javascript:;>>
> Message-ID:
>        <1340035297.45993.YahooMailNeo <@t> web121406.mail.ne1.yahoo.com<javascript:;>
> >
> Content-Type: text/plain; charset=iso-8859-1
>
> With the data you have from your employees, calculate the number of units
> per unit time you get for the different tasks.
> Feed your data on Excel and prepare totals per lab and per employee and
> make graphs that you can display and discuss with your boss and your
> employees.
> René J.
>
>
> ________________________________
> From: "Scott, Allison D" <Allison_Scott <@t> hchd.tmc.edu <javascript:;>>
> To: "histonet <@t> lists.utsouthwestern.edu <javascript:;>" <
> histonet <@t> lists.utsouthwestern.edu <javascript:;>>
> Sent: Monday, June 18, 2012 11:55 AM
> Subject: [Histonet] Employee productivity
>
> Hello to all in histoland.  How is employee productivity calculated.  I
> have my techs fill out a workload tally sheet daily.  My boss wants me to
> take the data and come up with a productivity chart or graph that says who
> is productive or not.  I don't need a tally sheet to say who is working or
> not.  Is any one doing this?  I need help.
>
>
> Allison Scott HT(ASCP)
> Histology Supervisor
> LBJ Hospital
> Houston, Texas 77026
>
> CONFIDENTIALITY NOTICE:
> If you have received this e-mail in error, please immediately notify the
> sender by return e-mail and delete this e-mail and any attachments from
> your computer system.
>
> To the extent the information in this e-mail and any attachments contain
> protected health information as defined by the Health Insurance Portability
> and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and
> 164; or Chapter 181, Texas Health and Safety Code, it is confidential
> and/or
> privileged.  This e-mail may also be confidential and/or privileged under
> Texas law.  The e-mail is for the use of only the individual or entity
> named
> above.  If you are not the intended recipient, or any authorized
> representative of the intended recipient, you are hereby notified that any
> review, dissemination or copying of this e-mail and its attachments is
> strictly prohibited.
>
> _______________________________________________
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> ------------------------------
>
> Message: 5
> Date: Mon, 18 Jun 2012 11:02:50 -0500
> From: Cheri Miller <cmiller <@t> physlab.com <javascript:;>>
> Subject: [Histonet] Fungus , GMS Aspergillus
> To: histonet <histonet <@t> lists.utsouthwestern.edu <javascript:;>>
> Cc: "histonet-bounces <@t> lists.utsouthwestern.edu <javascript:;>"
>        <histonet-bounces <@t> lists.utsouthwestern.edu <javascript:;>>
> Message-ID: <E3C81A010935EA41B379AC765103F3BF34348F60B8 <@t> olsrv12>
> Content-Type: text/plain; charset="us-ascii"
>
> I am looking for natural occurring Fungus, GMS Aspergillus to use as my
> fungus control tissue. Someone from Histonet gave me a block about 3 years
> ago. I've just used the last of it. Does anyone have any to share again?
>  Thanks Cheri
>
> Cheryl A. Miller HT(ASCP)cm
> Histology Supervisor
> Physicians Laboratory Services
> 4840 F Street
> Omaha, NE. 68127-0999
> 402 731 4145 ext. 554
>
>
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