[Histonet] Frozen Sections Falling Off Slides

[Ingles Claire]

Just remember - No formalin, no need for retrieval. In my experience that is the hardest step on tissue sections. It also shortens the procedure a bunch too. 
Claire

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Emily Sours
Sent: Sun 6/17/2012 2:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Frozen Sections Falling Off Slides



Wow you can run frozen sections through those machines? That's awesome.  I
figured it wouldn't be gentle enough to process them.
I'm a fan of Fisher Scientific superfrost plus slides.  Fisher claims the
slides are electrostatically charged to keep the sections from falling
off.  Whether that's a bunch of hooha or it's actually true, our sections
don't fall off, even after in situs.  I believe most scientific suppliers
sell their own brand of superfrost plus slides if you don't want to buy
from Fisher.

Emily

"You see a peanut, day's off to a good start; you witness some soil it's a
jamboree for Vince Noir."
--Howard Moon, in "Charlie", The Mighty Boosh



On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio <one_angel_secret <@t> yahoo.com>wrote:

> Was your leica set that cold? Maybe you could try warming the slide for a
> couple seconds before putting in alcohol. As far a charged slides I am
> amazed with the slides Dako sells for their IHC.
>
> Sent from my iPhone
>
> On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario <badzrosari <@t> yahoo.com>
> wrote:
>
> > Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen
> section Machine...But i dont understand why i got problems sections falling
> off slides.I used different types of slides non adhesive,adhesive(poly l
> lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken
> from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative
> etc..then after distilled water wash out...sections started to peel
> off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of
> tissue- fibroma and salivary gland...
> > I used Leica cryostat previously but i never got these problems...Even i
> used non adhesive but sections still on the slides..Can someone advised
>  please.Maybe youre using the same tissue tek cryo 3...
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