[Histonet] Frozen Sections Falling Off Slides

[Badz Yahoo]

Hello netters...have someone tried doin frozen in a tissue mistakenly immersed in formalin??not so long but the inside still fresh..m sure the slides will wash out..fresh always better..

On Jun 17, 2012, at 10:34 PM, Emily Sours <talulahgosh <@t> gmail.com> wrote:

> Wow you can run frozen sections through those machines? That's awesome.  I
> figured it wouldn't be gentle enough to process them.
> I'm a fan of Fisher Scientific superfrost plus slides.  Fisher claims the
> slides are electrostatically charged to keep the sections from falling
> off.  Whether that's a bunch of hooha or it's actually true, our sections
> don't fall off, even after in situs.  I believe most scientific suppliers
> sell their own brand of superfrost plus slides if you don't want to buy
> from Fisher.
> 
> Emily
> 
> "You see a peanut, day's off to a good start; you witness some soil it's a
> jamboree for Vince Noir."
> --Howard Moon, in "Charlie", The Mighty Boosh
> 
> 
> 
> On Sat, Jun 16, 2012 at 12:12 PM, Kim Donadio <one_angel_secret <@t> yahoo.com>wrote:
> 
>> Was your leica set that cold? Maybe you could try warming the slide for a
>> couple seconds before putting in alcohol. As far a charged slides I am
>> amazed with the slides Dako sells for their IHC.
>> 
>> Sent from my iPhone
>> 
>> On Jun 16, 2012, at 12:01 PM, Bernadette del Rosario <badzrosari <@t> yahoo.com>
>> wrote:
>> 
>>> Hello histonetters!!!Im presently using the Tissue Tek Cryo3 Frozen
>> section Machine...But i dont understand why i got problems sections falling
>> off slides.I used different types of slides non adhesive,adhesive(poly l
>> lysine,thermoshandon,starfrost sakura)..Regular procedure : Sections taken
>> from 2-4 microns,transfered to 95% alcohol fixative, wolfsgang fixative
>> etc..then after distilled water wash out...sections started to peel
>> off...My machine setting- chamber is -30 C;cryochamber is -39 C..example of
>> tissue- fibroma and salivary gland...
>>> I used Leica cryostat previously but i never got these problems...Even i
>> used non adhesive but sections still on the slides..Can someone advised
>> please.Maybe youre using the same tissue tek cryo 3...
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