[Histonet] anti-GFP staining rabbit polyclonal A11122

[Paula Pierce]

I use this exact antibody routinely at 1:1000.


Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com


________________________________
From: Marina Pils <Marina.Pils <@t> helmholtz-hzi.de>
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Monday, February 27, 2012 6:05 AM
Subject: [Histonet] anti-GFP staining rabbit polyclonal A11122

Dear all,
We are trying to establish an anti-GFP staining with rabbit polyclonal A11122 from Invitrogen.
We are using formalin fixed, paraffin embedded mouse tissue and want to distinguish between a GFP transgenic mouse strain and a wt mouse.
The problem is, we get a strong background in the wt, especially in spleen and lymphnodes, while the staining looks fine in liver.
We dilute the antibody 1:400 and use 2 min antigen retrieval in Citrate buffer.
Can anyone recommend a protocol? Or any other antibody that is working better?

Thanks a lot for your help,

Marina
________________________________________
Marina Pils, DVM, PhD
Animal experimental unit
Helmholtz Centre for Infection Research
Inhoffenstr. 7
D 38124 Braunschweig


________________________________

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