[Histonet] anti-GFP staining rabbit polyclonal A11122

[Marina Pils]

Dear all,
We are trying to establish an anti-GFP staining with rabbit polyclonal A11122 from Invitrogen.
We are using formalin fixed, paraffin embedded mouse tissue and want to distinguish between a GFP transgenic mouse strain and a wt mouse.
The problem is, we get a strong background in the wt, especially in spleen and lymphnodes, while the staining looks fine in liver.
We dilute the antibody 1:400 and use 2 min antigen retrieval in Citrate buffer.
Can anyone recommend a protocol? Or any other antibody that is working better?

Thanks a lot for your help,

Marina
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Marina Pils, DVM, PhD
Animal experimental unit
Helmholtz Centre for Infection Research
Inhoffenstr. 7
D 38124 Braunschweig


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