[Histonet] Cutting Tendon

[Rene J Buesa]

First of all fixation has to be perfect, if with NBF, at least 72 hours. Then increase twice your standard processing times, specially infiltration. Substitute your normal paraffin was to one of 63-65ºC and chill be blocks before sectioning.
René J.

--- On Fri, 2/17/12, Pablo Sanchez-Quinteiro <pablo.sanchez <@t> usc.es> wrote:


From: Pablo Sanchez-Quinteiro <pablo.sanchez <@t> usc.es>
Subject: [Histonet] Cutting Tendon
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, February 17, 2012, 6:13 AM



   Dear listers,
   I am trying to get transversal sections of cow flexor digital tendons.
   I  need  sections  of  the  whole  piece,  about  1cm diameter. Before
   paraffin embedding I have treated the samples with:
   -        KOH 4%
   -        4% phenol in 70% ethanol
   In both cases the results are very poor; the tissue is torn to pieces.
   I would very grateful for any input regarding this issue.
   Best Regards
   Pablo
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