[Histonet] Cutting Tendon

Pablo Sanchez-Quinteiro pablo.sanchez <@t> usc.es
Fri Feb 17 05:13:32 CST 2012


   Dear listers,
   I am trying to get transversal sections of cow flexor digital tendons.
   I  need  sections  of  the  whole  piece,  about  1cm diameter. Before
   paraffin embedding I have treated the samples with:
   -        KOH 4%
   -        4% phenol in 70% ethanol
   In both cases the results are very poor; the tissue is torn to pieces.
   I would very grateful for any input regarding this issue.
   Best Regards
   Pablo


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