[Histonet] (no subject)

[Miha Tesar]

Hi!
I am working in Institute of Phatology Slovenia EU. Recently I have problems with muscle biopsy. There are a lots of artefact that I can not explain, like little holes in the tissue after the tissue is being frozen. We are using the isopentan and the liquid nitrogen for the freezing. Can enyone give me some ideas haw to avoid this artefact. How important is humidity in laboratory for the good results?
The next problem is that after I put the tissue on the microscope slides after coloring the GTK all the tissue gets wrinkeld. Iam using the poly L lisin microscope slides or the Immuno microscope slides.
Thx in advance!
Best regards Miha from SLO