[Histonet] Re: Regarding cartilage and bone staining

[Kraniofacial Biology]

Hello
I would like to ask how many time do you put the mice head in xylene after
series of Ethanol wash in the way of making paraffin block. I also did all
the steps as well, but for embryonic day 17-18, even after observing
clearing in xylene and moving to the several times in Gurr/paraplast I find
the head to be soft and the initial part of head from which we start to cut
is white and when I removed the flecks of wax and touched it was still soft.
I am not getting what to do.
Pathik

On Tue, Oct 11, 2011 at 11:01 PM, Kraniofacial Biology <
kraniofacialbiology <@t> gmail.com> wrote:

> Hello Histonetters
> I am new to this site. I am a clinician and very fresh to research. I was
> doing whole mount cartilage and bone staining of whole head of mice and was
> surprised to see whole mouse head being used for experiment. I was wondering
> if there is any other staining method which can be used in place of the
> whole mount cartilage and bone staining that would stain the sections on a
> slide and works on cartilage and bone. This would also save the whole mouse
> to be used and many slides can also be used for other experiments.
> Regards
> Pathik
>