[Histonet] Cutting frozen sections from visceral fat

[abright <@t> brightinstruments.com]

You need to be at or below -28 degs C, also make sure that your knife and anti-roll plate are at or below this temperature too.
Best regards

Alan Bright
Bright Instrument Co. Ltd.
St Margarets Way
Huntingdon
Cambridgeshire 
PE29 6EU
www.brightinsruments.com

Sent from my BlackBerry® wireless device

-----Original Message-----
From: <idimitro <@t> mun.ca>
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
Date: Thu, 6 Oct 2011 11:58:53 
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] Cutting frozen sections from visceral fat

Hi everyone,

I am having trouble cutting frozen sections from visceral fat. I have never cut only fat before. I am also cutting liver sections and they are perfect, but when I try to cut the fat it is not working.
The temperature of the cryostat is ( -20) degree Celsius.
I wonder if anyone has ever done this. I was thinking that if I lower the temperature or fix the fat with formol-calcium I may have success.

Iliana Dimitrova, MSc, RT
Histology Supervisor
Room 2808
Medical Education and Laboratory Support Services (MELSS)
Faculty of Medicine
Memorial University
St. John's, NL


This electronic communication is governed by the terms and conditions at
http://www.mun.ca/cc/policies/electronic_communications_disclaimer_2011.php
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


-- 
BEGIN-ANTISPAM-VOTING-LINKS
------------------------------------------------------


Teach SpamSniper if this mail (ID 01FFoF7eL) is spam:

Spam:        http://admin.spamsniper.co.uk/canit/b.php?i=01FFoF7eL&m=bc910a912ee7&t=20111006&c=s

Not spam:    http://admin.spamsniper.co.uk/canit/b.php?i=01FFoF7eL&m=bc910a912ee7&t=20111006&c=n

Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01FFoF7eL&m=bc910a912ee7&t=20111006&c=f
------------------------------------------------------
END-ANTISPAM-VOTING-LINKS