[Histonet] Cutting frozen sections from visceral fat

[idimitro <@t> mun.ca]

Hi everyone,

I am having trouble cutting frozen sections from visceral fat. I have never cut only fat before. I am also cutting liver sections and they are perfect, but when I try to cut the fat it is not working.
The temperature of the cryostat is ( -20) degree Celsius.
I wonder if anyone has ever done this. I was thinking that if I lower the temperature or fix the fat with formol-calcium I may have success.

Iliana Dimitrova, MSc, RT
Histology Supervisor
Room 2808
Medical Education and Laboratory Support Services (MELSS)
Faculty of Medicine
Memorial University
St. John's, NL


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