[Histonet] Re: Histonet Digest, Vol 95, Issue 1
Gmicek
gmicek <@t> wowway.com
Sat Oct 1 19:35:10 CDT 2011
Make your own with moldy bread. Works great!
G Micek
Sent from my iPhone
On Oct 1, 2011, at 12:07 PM, histonet-request <@t> lists.utsouthwestern.edu wrote:
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> Today's Topics:
>
> 1. Control Tissue (Terry&Star Bullard)
> 2. Position Available @ NIH (Bethesda, MD) (Jason Blaine)
> 3. Histonet Digest, Vol 94, Issue 40 Nothing down the drain
> (Steve McClain)
> 4. washing the placenta (An Eerdekens)
> 5. Microtome Alignment Tool (Marcia Fisher)
> 6. RE: AFB Controls (Farish, Craig)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 30 Sep 2011 13:29:43 -0400
> From: "Terry&Star Bullard" <tnsbull <@t> gmail.com>
> Subject: [Histonet] Control Tissue
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CADvTC3zm+CQj-6fk_LUK_3NLewd_9wb6YoQ5QEVWvBzJ-purSg <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi all. Does anyone have a resource for control blocks? We are in need
> of fungus controls and are trying to save the cost of buying slides.
> Thanks all.
>
> Sheila Haas
> Laboratory Supervisor
> MicroPath Laboratories, Inc
>
>
> Sheila,
> National Society for Histotechnology has a Tissue Control Bank, If you
> contact the person below she can help:
>
> Melinda A Hamilton HT (ASCP)
> NSH Tissue Control Bank Curator
> Melinda Sokol <m.sokol37 <@t> gmail.com>
>
> Hope that help!
> Star Bullard HT(ASCP)
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 30 Sep 2011 17:33:27 +0000
> From: Jason Blaine <jblaine <@t> astrixinc.com>
> Subject: [Histonet] Position Available @ NIH (Bethesda, MD)
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <A115DD9FF6F4A14D8DCF77948DCE580C74C98E <@t> MBX021-W3-CA-1.exch021.domain.local>
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>
> Seeking highly skilled scientist or histologist who is well versed in IHC. This position is in the National Institute of Mental Health (NIMH) and thus requires prior neuroscience and non-human primate experience. Please note that we are NOT necessarily seeking ASCP histology certificated individuals and that prior related experience will be preferred over certification.
>
> The position will support research in the NIMH. The position will provide histology support service for the complete laboratory (has 5 sections within it). This person will be the go to person for histology and IHC within the lab and its 5 sections. This individual will be expected to function as a resource to all of the lab's not solely as a technician but rather as a histology/IHC expert to advise the teams with their histology/IHC needs. This will be a collaborative research effort and will not offer personal independent research. Non-human primate model is the principal concern. Experience with IHC, Fluoro-ruby and green, D2, BrDu, tyrosine hyroxylase, perfusion, brain removal and prep, and much more is desired.
>
> If this opportunity is not right for you I ask that you point me in the direction of someone who may be appropriately qualified - maybe a colleague of yours, or former boss, or collaborator. The opportunity is a fulltime, salaried, indefinite, contractor position with benefits.
>
> Qualified candidates please submit a current CV/resume (in MS Word format) to jblaine <@t> astrixinc.com.
>
> Thanks -
> Jason Blaine
> jblaine <@t> astrixinc.com
>
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 30 Sep 2011 14:08:27 -0400
> From: "Steve McClain" <SteveM <@t> mcclainlab.com>
> Subject: [Histonet] Histonet Digest, Vol 94, Issue 40 Nothing down the
> drain
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <EE723B268CDC7F4E85D42139B6F5416337B061 <@t> ml1.McClainLabs.local>
> Content-Type: text/plain; charset="us-ascii"
>
> Yes nothing down the drain (except maybe the lithium carbonate bluing).
>
> Hematoxylin goes into medical waste service.
>
> Used Eosin gets reused a 10-20 ml at a time into the first absolute on
> the processors as a marker of carryover (as the later alcohols turn red,
> we have a visual indicator of the need to change, also helps with
> embedding since collagen stains more than epithelium/epidermis).
>
> Alcohols after the de-waxing xylenes and from the processor purges go to
> the medical waste service.
>
> Other alcohols get recycled.
>
> All the Xylenes get recycled.
>
> Liquid Waste from the recycler goes to medical waste service.
>
> Paraffins go into the trash.
>
>
>
>
>
> Steve
>
> Steve A. McClain, MD
>
> McClain Labs, LLC 45 Manor Road, Smithtown, NY 11787 631 361 4000
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 30 Sep 2011 21:18:10 +0200
> From: An Eerdekens <an.eerdekens <@t> uzleuven.be>
> Subject: [Histonet] washing the placenta
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <09F2BA9A1E06C64D9F090FF0FD0D73179763AFDBC7 <@t> EX2007-MBX-2.uz.kuleuven.ac.be>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Dear all,
>
> Is there someone with experience in washing of placentae before sampling?
>
> Regards
>
> Dr An Eerdekens
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 30 Sep 2011 19:33:59 +0000
> From: Marcia Fisher <mfisher <@t> ecrmc.org>
> Subject: [Histonet] Microtome Alignment Tool
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <BAC5284D8BDC9B40819E2C75ED34D065188932 <@t> EXMBX01.ecrmc.ci.el-centro.ca.us>
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> Content-Type: text/plain; charset="us-ascii"
>
> We just purchased one from American Master Tech and really like the results. We now have added this task to our monthly maintenance sheets.
>
> Marcia Fisher
> Histology Supervisor/Lab Safety Officer
> El Centro Regional Medical Center
>
>
> ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments.
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>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Sat, 1 Oct 2011 20:57:25 +1000
> From: "Farish, Craig" <CFarish <@t> csu.edu.au>
> Subject: [Histonet] RE: AFB Controls
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <3232733C82C90D439BF48C9FEF3B7D1C2F78512D98 <@t> MAIL01.CSUMain.csu.edu.au>
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>
> Hi Pamela, try contacting your local large animal vet or a veterinary diagnostic lab. Any case of Ovine or Bovine Johnne's disease would be a great ZN positive control. The bacteria (mycobacterium avium subsp paratuberculosis) is acid fast and congregates in huge numbers in the terminal ileum and ileo-caecal junction (and also the caudal mesenteric lymph node in smaller numbers).
> About the only problem i've ever come across is that there are so many AFBs it may mask poorly stained section unless you look for individual bacteria in the margins.
> Hope this helps
> Craig
>
> Craig Farish
> Senior TO
> Veterinary Diagnostic Laboratory
> Charles Sturt University
> Wagga Wagga
> NSW, Australia
>
>
> ------------------------------
>
> Message: 12
> Date: Thu, 29 Sep 2011 18:23:07 +0000
> From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
> Subject: [Histonet] AFB Controls
> To: "histonet <@t> lists.utsouthwestern.edu"
> <histonet <@t> lists.utsouthwestern.edu>
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> <41D3A1AF6FEF0643BDC89E0516A6EA3209697449 <@t> Mail2Node2.ad.uams.edu>
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>
> Does anyone know where we can get a good AFB control? Ours have exhausted and we can't find a case here with a positive reading.
>
> Best Regards,
>
> Pamela A Marcum
> UAMS
> Slot 502
> 4301 W Markham Street
> Little Rock AR 72205
> Office: 501-686-7554
> Fax: 501-686-7151
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