[Histonet] RE: AFB Controls

[Farish, Craig]

Hi Pamela, try contacting your local large animal vet or a veterinary diagnostic lab. Any case of Ovine or Bovine Johnne's disease would be a great ZN positive control. The bacteria (mycobacterium avium subsp paratuberculosis) is acid fast and congregates in huge numbers in the terminal ileum and ileo-caecal junction (and also the caudal mesenteric lymph node in smaller numbers).
About the only problem i've ever come across is that there are so many AFBs it may mask poorly stained section unless you look for individual bacteria in the margins.
Hope this helps
Craig

Craig Farish
Senior TO
Veterinary Diagnostic Laboratory
Charles Sturt University
Wagga Wagga
NSW, Australia


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Message: 12
Date: Thu, 29 Sep 2011 18:23:07 +0000
From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
Subject: [Histonet] AFB Controls
To: "histonet <@t> lists.utsouthwestern.edu"
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Does anyone know where we can get a good AFB control?  Ours have exhausted and we can't find a case here with a positive reading.

Best Regards,

Pamela A Marcum
UAMS
Slot 502
4301 W Markham Street
Little Rock AR 72205
Office: 501-686-7554
Fax: 501-686-7151

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