[Histonet] Verhoeff/Masson's Stain

[Liz Chlipala]

John

We do this stain all of the time, but we make up our reagents from
scratch we don't use a kit.  My suggestion is to make up your elastic
stain fresh.  We make up our elastic stain fresh and use it only once
(or for that day) and then discard.  I have attached our procedure in a
different e-mail.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
www.premierlab.com
 
 
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John
Shelley
Sent: Tuesday, March 22, 2011 11:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Verhoeff/Masson's Stain

Hello Histonetters,

I have been asked to work on a project that will require me to do a
Verhoeff/Masson's stain. I have run some samples through the stain from
a protocol that I found on the archives and it is not working to my
satisfaction. I am not getting the nice elastin fibers to show black
like they should. I have looked at the slides just before going into 2%
ferric chloride and it appears to be dull or limited staining. I am
using a kit from PolyScientific and not sure if that might be my problem
or not. My question is, are there another people who are running this
special stain combo and would you be willing to share the procedure and
also who you are buying your reagents from to accomplish the staining. I
appreciate any help with this request. Thanks!!!

Kind Regards!

John J Shelley


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