[Histonet] Verhoeff/Masson's Stain

[John Shelley]

Hello Histonetters,

I have been asked to work on a project that will require me to do a Verhoeff/Masson's stain. I have run some samples through the stain from a protocol that I found on the archives and it is not working to my satisfaction. I am not getting the nice elastin fibers to show black like they should. I have looked at the slides just before going into 2% ferric chloride and it appears to be dull or limited staining. I am using a kit from PolyScientific and not sure if that might be my problem or not. My question is, are there another people who are running this special stain combo and would you be willing to share the procedure and also who you are buying your reagents from to accomplish the staining. I appreciate any help with this request. Thanks!!!

Kind Regards!

John J Shelley