[Histonet] PFA preparation

[Sherwood, Margaret]

We also purchase a 4L cubitainer of 10% buffered formalin from Fisher. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Merced Leiker
Sent: Monday, March 21, 2011 12:44 PM
To: Montina Van Meter; Madary, Joseph; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] PFA preparation

I concur with the others. For about 6 years I've made it from powder but 
the toxicity risk bothered me until I realized how safely and cheaply you 
can just buy the same stuff. We get a discount through our stockroom on a 
4L bottle of 10% Buffered Formalin from Fisher (SF100-4) for $28.


I started getting neutral buffered formalin instead from Fisher.

--On Monday, March 21, 2011 11:25 AM -0500 Montina Van Meter 
<Montina.VanMeter <@t> pbrc.edu> wrote:

> Nick,
>
> I too have tried to limit my exposure after making it from scratch for
> 30+ years.  I currently purchase it from USB Corp. located in Cleveland
> Ohio. They have been acquired by Affymetrix and you will be directed to
> that website when you type in www.usbweb.com.
>
> Cat.# 19943 1LT   Paraformaldehyde Solution, 4% in PBS, 1L  $48.00
>
>
> Regards,
> Tina
>
>
>
>
>
> Montina J. Van Meter
> Lab Manager
> Autonomic Neuroscience
> 225-763-2564
> vanmetmj <@t> pbrc.edu
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Madary,
> Joseph
> Sent: Monday, March 21, 2011 10:27 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] PFA preparation
>
> Anyone have a decent method to prepare paraformaldehyde.  I have the
> powder and the NOAH, hood, heat source but forgot the method.
>
>
> Nick Madary, HT/HTL(ASCP)QIHC
>
> Histology Mgr, Medimmune
>
> 301.398.6360(lab), 4745(vm),9745(fax)
>
>
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Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

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