[Histonet] PFA preparation

[Merced Leiker]

I concur with the others. For about 6 years I've made it from powder but 
the toxicity risk bothered me until I realized how safely and cheaply you 
can just buy the same stuff. We get a discount through our stockroom on a 
4L bottle of 10% Buffered Formalin from Fisher (SF100-4) for $28.


I started getting neutral buffered formalin instead from Fisher.

--On Monday, March 21, 2011 11:25 AM -0500 Montina Van Meter 
<Montina.VanMeter <@t> pbrc.edu> wrote:

> Nick,
>
> I too have tried to limit my exposure after making it from scratch for
> 30+ years.  I currently purchase it from USB Corp. located in Cleveland
> Ohio. They have been acquired by Affymetrix and you will be directed to
> that website when you type in www.usbweb.com.
>
> Cat.# 19943 1LT   Paraformaldehyde Solution, 4% in PBS, 1L  $48.00
>
>
> Regards,
> Tina
>
>
>
>
>
> Montina J. Van Meter
> Lab Manager
> Autonomic Neuroscience
> 225-763-2564
> vanmetmj <@t> pbrc.edu
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Madary,
> Joseph
> Sent: Monday, March 21, 2011 10:27 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] PFA preparation
>
> Anyone have a decent method to prepare paraformaldehyde.  I have the
> powder and the NOAH, hood, heat source but forgot the method.
>
>
> Nick Madary, HT/HTL(ASCP)QIHC
>
> Histology Mgr, Medimmune
>
> 301.398.6360(lab), 4745(vm),9745(fax)
>
>
> "To the extent this electronic communication or any of its attachments
> contain information that is not in the public domain, such information
> is considered by MedImmune to be confidential and proprietary, and
> expected to be used only by the individual(s) for whom it is intended.
> If you have received this electronic communication in error, please
> reply to the sender advising of the error in transmission and delete the
> original message and any accompanying documents from your system
> immediately, without copying, reviewing or otherwise using them for any
> purpose.  Thank you for your cooperation."
>
>
> To the extent this electronic communication or any of its attachments
> contain information that is not in the public domain, such information
> is considered by MedImmune to be confidential and proprietary. This
> communication is expected to be read and/or used only by the
> individual(s) for whom it is intended. If you have received this
> electronic communication in error, please reply to the sender advising
> of the error in transmission and delete the original message and any
> accompanying documents from your system immediately, without copying,
> reviewing or otherwise using them for any purpose. Thank you for your
> cooperation.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



Merced M Leiker
Research Technician II
354 Biomedical Research Building
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.