[Histonet] immunostaining question

[Sebree Linda A]

Yes, most definitely. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pop
Elena
Sent: Monday, March 14, 2011 3:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] immunostaining question

Hello everybody,

I am trying some new antibodies on parafin embeded radical
prostactectomies 
tissue sections and I started with the highest recomended dilution but
it seems 
I did not get any staining. I already counterstained the slides w/
hematoxylin 
and I was wondering if it's possible to go a few steps back and incubate
again 
with a higher concentration of the antibody? I have a limited amount of
tissue 
sections for "testing".

Any imput is appreciated!
Thanks,
Leni


      
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