[Histonet] Polymer detection kit

[sgoebel <@t> mirnarx.com]

I use the Biocare polymers all the time because they are cheaper than
DAKO.  Some of them are biotin free, so as long as your primary has no
biotin and you aren't staining anything with endogenous biotin you can
omit that block.  Other than that enzyme and protein blocking I still
do.  Some of them are a two step process and some are one solution.
They work great!  Just follow the protocol included with the polymer =)
Good Luck!!

Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
amitapandey <@t> torrentpharma.com
Sent: Friday, March 11, 2011 4:54 AM
To: Histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: [Histonet] Polymer detection kit

Dear Histonet fellow,

I am planning to use  Bio care detection kit -MRT511 (for mouse
monoclonal 
antibody on rat kidney FFPE) and RMR622 (for rabbit polyclonal antibody
on 
rat kidneyFFPE)

Do i have to buy any additional reagents to perform the 
staining.(specially blocker or antigen retrieval)

Any feed back on their results and trouble shootings are welcome.
 
Amita
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