[Histonet] RE: Preferred green counterstain for GMS?

[Tony Henwood]

Ally,

I would use Fast Green FCF. It does not fade as much as Light Green. Use a 1% in 2% Acetic acid.
Tartrazine will give you a yellow colour.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Langsdorf, Aliete E.
Sent: Wednesday, 9 March 2011 3:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Preferred green counterstain for GMS?

Hi,

What is your preferred green counterstain for GMS?
In the kit I have, it suggests Fast Green FCF, Light Green SF Yellowish or Tartrazine.
Which should I buy?

Any help is appreciated!
Thanks!
~Ally

Senior Research Technician
Medical Oncology
Dana-Farber Cancer Institute




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