[Histonet] RE: Preferred green counterstain for GMS?

[Reilly, Laurie]

My mentors way back taught us to use Methyl Green as a counterstain for GMS because it also stains nuclei, but it has to be cleared with chloroform to remove Methyl Violet contamination.

Regards,   Laurie.

Mr. Laurie REILLY
Histopathology
School of  Veterinary and Biomedical Sciences
James Cook University
Townsville  Qld.  4811
Australia.

Phone 07 4781 4468

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Langsdorf, Aliete E.
Sent: Wednesday, 9 March 2011 2:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Preferred green counterstain for GMS?

Hi,

What is your preferred green counterstain for GMS?
In the kit I have, it suggests Fast Green FCF, Light Green SF Yellowish or
Tartrazine.
Which should I buy?

Any help is appreciated!
Thanks!
~Ally

Senior Research Technician
Medical Oncology
Dana-Farber Cancer Institute




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