[Histonet] processing times for skin specimens
Steve McClain
SteveM <@t> mcclainlab.com
Thu Apr 21 19:24:29 CDT 2011
90 minutes for up to 2mm thickness using K3000 or VIP5.
I do not have a VIP6
Steve A. McClain, MD
McClain Laboratories, LLC
45 Manor Road
Smithtown, NY 11787
631 361-4000
631 361-4037fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, April 21, 2011 12:51 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 89, Issue 24
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Today's Topics:
1. Cassette labeler (Martin, Gary)
2. 'Deplasticizing' before staining? (Mahesh Polavarapu)
3. Re: Request help on finding a part for Leitz 1512 (Henry Chidgey)
4. RELIA Histology Job Alert. Excellent opportunity on Long
Island Day shift NY Lic required. (Pam Barker)
5. RE: Re: Request help on finding a part for Leitz 1512
(Weems, Joyce)
6. Re: Re: Request help on finding a part for Leitz 1512
(Grantham, Andrea L - (algranth))
7. Part time Histo Tech position in Phoenix AZ (jcox90 <@t> yahoo.com)
8. Unsubscribe (Tate Maria)
9. Re: Unsubscribe (Mollie Hannon)
10. Graduating in June from histo program (Teresa Moore)
11. RE: whole retinal staining (Abbey Mortimer)
12. How to Unsubscribe (Lee & Peggy Wenk)
13. trichrome/ frozen cardiac tissue (Molinari, Betsy)
14. UCP1 Antibody (John Shelley)
15. IHC validation (Troutman, Kenneth A)
16. cyto prep (Cynthia Pyse)
17. processing times for skin specimens (Carol Bryant)
18. IHC menu (Houston, Ronald)
19. Re: cyto prep (Sheila Haas)
20. Re: Unsubscribe (Emily Sours)
21. RE: cyto prep (Weems, Joyce)
22. Re: Unsubscribe (Merced Leiker)
23. eosin bleeding on frozen sections (Christi Cosby)
24. Neutralizing Formalin (Evans, Andria B)
25. RE: eosin bleeding on frozen sections (sgoebel <@t> mirnarx.com)
26. (no subject) (SHANE NELSON)
----------------------------------------------------------------------
Message: 1
Date: Wed, 20 Apr 2011 10:12:34 -0700
From: "Martin, Gary" <gmartin <@t> marshallmedical.org>
Subject: [Histonet] Cassette labeler
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6ED9D4252F278841A0593D3D788AF24C0D480110 <@t> mailsvr.MARSHMED.local>
Content-Type: text/plain; charset="us-ascii"
We have just received a Lamb Micro Writer (model # E22.01MWI) single shoe. The problem is that we did not receive an operator manual. Does anyone know where I can get a manual?
------------------------------
Message: 2
Date: Wed, 20 Apr 2011 12:24:55 -0500
From: Mahesh Polavarapu <polavarapu.mahesh <@t> gmail.com>
Subject: [Histonet] 'Deplasticizing' before staining?
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BANLkTim6mC2iuyvCLeX5G=9FwSpcJRQeTA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi,
I have 50uM thick sections of soft tissue embedded in MMA (cold cured Technovit 9100). Do these need to be 'deplasticized' before they will take up stain? Thanks in advance!
- Mahesh
------------------------------
Message: 3
Date: Wed, 20 Apr 2011 12:31:10 -0500
From: "Henry Chidgey" <hchidgey <@t> earthlink.net>
Subject: [Histonet] Re: Request help on finding a part for Leitz 1512
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <016f01cbff80$bef19030$3cd4b090$@net>
Content-Type: text/plain; charset="us-ascii"
I was asked by a member to share the solution so here it is :
Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA.
He will probably have some or can make you some.
Great guy and very knowledgeable. Thanks Joyce for giving me this informationJ
Henry
------------------------------
Message: 4
Date: Wed, 20 Apr 2011 13:36:45 -0400
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA Histology Job Alert. Excellent opportunity
on Long Island Day shift NY Lic required.
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <DCFA9EE40E204519866E4B9E75992D67 <@t> ownerf1abaad51>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonetters!!
I have a great new opportunity that I want to tell you about. I am working with a Laboratory located on Long Island that is in need of a NY licensed histology tech. This is a dayshift full time permanent position. My client offers a very competitive salary, excellent benefits, a great place to work and they are ready to interview and hire right away. If you or anyone you know might be interested in this position please contact me. I can be reached toll free at 866-607-3542 or relia1 <@t> earthlink.net. Thanks and have a great day!!
Thank You!
Pam Barker
President
RELIA
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: relia1 <@t> earthlink.net
www.facebook.com search Pam Barker RELIA www.linkedin.com/reliasolutions www.myspace.com/pamatrelia www.twitter.com/pamatrelia
------------------------------
Message: 5
Date: Wed, 20 Apr 2011 14:54:58 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] Re: Request help on finding a part for Leitz
1512
To: "hchidgey <@t> earthlink.net" <hchidgey <@t> earthlink.net>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92AD9B20A6C38C4587A9FEBE3A30E164081F4BB962 <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
He worked in the Leica factory and is truly an expert.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Henry Chidgey
Sent: Wednesday, April 20, 2011 13:31
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Request help on finding a part for Leitz 1512
I was asked by a member to share the solution so here it is :
Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA.
He will probably have some or can make you some.
Great guy and very knowledgeable. Thanks Joyce for giving me this informationJ
Henry
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------------------------------
Message: 6
Date: Wed, 20 Apr 2011 12:51:48 -0700
From: "Grantham, Andrea L - (algranth)" <algranth <@t> email.arizona.edu>
Subject: Re: [Histonet] Re: Request help on finding a part for Leitz
1512
To: "hchidgey <@t> earthlink.net" <hchidgey <@t> earthlink.net>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <DAEBDD23-2A85-4F2A-84D3-11A82925D50C <@t> email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"
I second getting in touch with Klaus - he knows the 1512 inside and out.
Andi Grantham
On Apr 20, 2011, at 10:31 AM, Henry Chidgey wrote:
> I was asked by a member to share the solution so here it is :
>
> Call Klaus Dern - 706-635-8840 - Microprecision Instruments - Ellijay, GA.
> He will probably have some or can make you some.
>
> Great guy and very knowledgeable. Thanks Joyce for giving me this
> informationJ
>
> Henry
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 7
Date: Wed, 20 Apr 2011 13:08:11 -0700 (PDT)
From: jcox90 <@t> yahoo.com
Subject: [Histonet] Part time Histo Tech position in Phoenix AZ
To: "Histonet <@t> Lists. Utsouthwestern. Edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <58759.56388.qm <@t> web161609.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Part time Histo Tech position open with Southwest Skin Specialists, Ltd., located in Phoenix AZ. Responsibilities for this position include: grossing and fixation, processing, embedding, sectioning and staining. Education
Requirements: Associate Degree in Applied Science/Histology and/or Histologic Certificate and HT or HTL ASCP Certification. If interested please send resume to miverson <@t> swskin.net.
Jill Cox, HT ASCP
------------------------------
Message: 8
Date: Wed, 20 Apr 2011 16:36:01 -0700 (PDT)
From: Tate Maria <spanish_fly1981 <@t> yahoo.com>
Subject: [Histonet] Unsubscribe
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <383929.85727.qm <@t> web125706.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
please remove from this mailing list
--- On Thu, 4/7/11, ti fei <tifei <@t> foxmail.com> wrote:
From: ti fei <tifei <@t> foxmail.com>
Subject: [Histonet] Unsubscribe
To: "Vanessa Avalos" <vavalos <@t> allergydermatology.com>, "HISTONET LISTS"
Date: Thursday, April 7, 2011, 9:26 AM
Hi please remove me from the mailing list
------------------ Original ------------------
From: "Vanessa Avalos"<vavalos <@t> allergydermatology.com>;
Date: Thu, Apr 7, 2011 05:54 AM
To: "HISTONET LISTS"<histonet <@t> lists.utsouthwestern.edu>;
Subject: RE: [Histonet] Slides sent out for outside consult.
Our practice does not bill the patient. Before sending they sign a waiver giving us permission to send out. On this waiver it states that if this is something their insurance does not cover then they may revieve a bill for $-$ range. We have an agreement with our consulting lab that they will only accept what the individual insurance covers. Our patients were getting ridiculous bills for consulting and our office was getting angry phone calls. After talking to the lab they made this agreement.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Wednesday, April 06, 2011 2:36 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slides sent out for outside consult.
How is everyone handling a case that you send out to an outside consultant as far as billing the patient for the consult. Right now if I send out a case they bill me back an 88321. I am being told that I can not pass this same 88321 on to the patient. Having the outside consultant do the billing is not an option.
Thanks,
Mike
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Message: 9
Date: Wed, 20 Apr 2011 20:21:18 -0400
From: Mollie Hannon <mollie <@t> phylogenyinc.com>
Subject: Re: [Histonet] Unsubscribe
To: histonet <@t> lists.utsouthwestern.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <81BCDB6F-2770-4C4B-833C-A55143E09806 <@t> phylogenyinc.com>
Content-Type: text/plain; charset=us-ascii
Please remove me from this list. Thank you.
On Apr 20, 2011, at 7:36 PM, Tate Maria wrote:
>
> please remove from this mailing list
> --- On Thu, 4/7/11, ti fei <tifei <@t> foxmail.com> wrote:
>
> From: ti fei <tifei <@t> foxmail.com>
> Subject: [Histonet] Unsubscribe
> To: "Vanessa Avalos" <vavalos <@t> allergydermatology.com>, "HISTONET LISTS"
> Date: Thursday, April 7, 2011, 9:26 AM
>
> Hi please remove me from the mailing list
> ------------------ Original ------------------
> From: "Vanessa Avalos"<vavalos <@t> allergydermatology.com>;
> Date: Thu, Apr 7, 2011 05:54 AM
> To: "HISTONET LISTS"<histonet <@t> lists.utsouthwestern.edu>;
>
> Subject: RE: [Histonet] Slides sent out for outside consult.
>
>
> Our practice does not bill the patient. Before sending they sign a waiver
> giving us permission to send out. On this waiver it states that if this is
> something their insurance does not cover then they may revieve a bill for
> $-$ range. We have an agreement with our consulting lab that they will only
> accept what the individual insurance covers. Our patients were getting
> ridiculous bills for consulting and our office was getting angry phone
> calls. After talking to the lab they made this agreement.
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Pence
> Sent: Wednesday, April 06, 2011 2:36 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Slides sent out for outside consult.
>
> How is everyone handling a case that you send out to an outside
> consultant as far as billing the patient for the consult. Right now if I
> send out a case they bill me back an 88321. I am being told that I can
> not pass this same 88321 on to the patient. Having the outside
> consultant do the billing is not an option.
>
> Thanks,
> Mike
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> -----Inline Attachment Follows-----
>
> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Wed, 20 Apr 2011 22:30:18 -0400
From: Teresa Moore <tmoore9k <@t> gmail.com>
Subject: [Histonet] Graduating in June from histo program
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BANLkTim2WAiL9NkW2dQWfZD3VyR6LQEbbQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I will be graduating from the histology program at Columbus State Community
College in June. Looking for a histotechnician position in or near
Columbus, Ohio. Any advice or leads are appreciated.
Thank you.
Teresa Moore
------------------------------
Message: 11
Date: Thu, 21 Apr 2011 12:48:46 +1000
From: Abbey Mortimer <A.Mortimer <@t> latrobe.edu.au>
Subject: [Histonet] RE: whole retinal staining
To: "David A. Wright" <dw18 <@t> uchicago.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E44363415887E640B905F07380B6872F1A1FB9B19D <@t> EXCH2007.ltu.edu.au>
Content-Type: text/plain; charset="iso-8859-1"
Thank you all so very much!
Abbey Mortimer
PhD Candidate
School of Psychological Science
La Trobe University
Bundoora, Victoria, 3083
AUSTRALIA
Phone: (03) 9479 2470
Email: a.mortimer <@t> latrobe.edu.au
________________________________________
From: David A. Wright [dw18 <@t> uchicago.edu]
Sent: Wednesday, 20 April 2011 7:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Cc: Abbey Mortimer
Subject: whole retinal staining
Dear Abbey & Histonet
I agree with René Buesa: I would try to stain whole retinae by a 'floating section' method i.e. hand processing in a dipping tray or mesh baskets (brush transfer can be done too but is laborious). Then mount them at the end.
Floating allows reagents to penetrate from both sides of the retina - extra valuable because your incubation times are going to be very long with material this thick. (I assume you are not just interested in superficial layers.) A microwave processor would help with penetration, but I have no direct experience.
-David
==
David A. Wright, Ph.D.
University of Chicago
Section of Neurosurgery
ORIGINAL POST:
Histonet Digest, Vol 89, Issue 21 Message: 7
Date: Tue, 19 Apr 2011 19:00:27 +1000
From: Abbey Mortimer <A.Mortimer <@t> latrobe.edu.au>
Subject: [Histonet] Tissue adhesion to slides
Hello out there!
I was wondering if any of you have experience in attaching human retinae (~250-300 microns thick) to slides for manual immunohistochemistry. Starfrost does not seem adequate.
Any advice would be greatly appreciated!
Regards,
Abbey
Abbey Mortimer
PhD Candidate
School of Psychological Science
La Trobe University
Bundoora, Victoria, 3083
AUSTRALIA
Phone: (03) 9479 2470
Email: a.mortimer <@t> latrobe.edu.au
------------------------------
Message: 12
Date: Thu, 21 Apr 2011 05:48:41 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: [Histonet] How to Unsubscribe
To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1EA881612BC34D52BB4B1C32ADA42CCE <@t> HP2010>
Content-Type: text/plain; charset="iso-8859-1"
It's a do-it-yourself.
- Scroll to bottom of any Histonet email.
- Click on the link that has the word "lists" in it.
- Scroll to the bottom of that page, where there is information on how to unsubscribe.
- Follow directions.
Peggy Wenki
------------------------------
Message: 13
Date: Thu, 21 Apr 2011 12:25:24 +0000
From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>
Subject: [Histonet] trichrome/ frozen cardiac tissue
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D0902470CECC5A45AEC43366C474B62C349B15 <@t> THIEXCH02.THI2.com>
Content-Type: text/plain; charset="us-ascii"
Hi,
I have some frozen house hearts that were perfused with 4% paraformaldehyde. I found protocols for FF, but not fixed frozen. I tried staining them with my usual Gomori I use on my paraffin blocks but the doc thinks they picked up too much red, not enough green.
Any suggerstions..I need to get them done today, they were brought up to RT this morning.
Thanks.
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
MC1-283
Houston,TX 77030-2607
832-355-6524
832-355-6812
------------------------------
Message: 14
Date: Thu, 21 Apr 2011 09:13:39 -0400
From: John Shelley <jshelley <@t> sanfordburnham.org>
Subject: [Histonet] UCP1 Antibody
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A0E1854154C4BF4C9EF42A735FD19C741587E6FF0F <@t> LN-MAIL07.ln.burnham.org>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonetters,
I was hoping those of you who may have worked with UCP1 antibody might be willing to share how you optimized this antibody. I am using UCP1 antibody (ab10983) from Abcam. I have followed the datasheet and have used both protease and proteinase k and at varying times with no results. I am using brown fat from a wild type mouse and this is the control tissue that should work. I am running it overnight in a 4 ° to see if that might help since the Post Doc said that their western blot worked better that way. Any help or ideas how to proceed would be appreciated. Thanks in advance!
Kind Regards!
John J Shelley
------------------------------
Message: 15
Date: Thu, 21 Apr 2011 08:16:39 -0500
From: "Troutman, Kenneth A" <Ashley.Troutman <@t> Vanderbilt.Edu>
Subject: [Histonet] IHC validation
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<7B310892042DA74CB3590053F424CFE613D63CD2A5 <@t> ITS-HCWNEM06.ds.Vanderbilt.edu>
Content-Type: text/plain; charset="us-ascii"
Hematoxylin should not affect the immunoreactivity (since counterstaining occurs following the IHC reaction), so no, changing the hematoxylin would not require a revalidation. Just make sure you are not obscuring the immunostain with an overly-dark counterstain. The point is to provide contrast between the DAB (brown) or fast red chromagen and to offer the reader a sense of context of the surrounding morphology of the tissue being stained.
Ashley Troutman BS, HT(ASCP) QIHC
Immunohistochemistry Supervisor
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4531
Nashville, TN 37232
Message: 10
Date: Tue, 19 Apr 2011 14:04:32 -0500
From: "Ring, Mary L" <Mary.L.Ring <@t> HealthPartners.Com>
Subject: [Histonet] IHC validation
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2594D39B69223047B6E2F4ADF1E62DB9010FFB487841 <@t> HPEMX3.HealthPartners.int>
Content-Type: text/plain; charset="iso-8859-1"
Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains?
Thanks for your help!
Mary Ring, HT, QIHC
Regions Hospital, St Paul, Mn
------------------------------
Message: 16
Date: Thu, 21 Apr 2011 09:51:35 -0400
From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
Subject: [Histonet] cyto prep
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001501cc002b$3a929360$afb7ba20$@com>
Content-Type: text/plain; charset="us-ascii"
Good Morning Histonetters
For those of you who have pap smears in their facility's, who do you have
preparing the pap smears. Lab assistants, lab tech, or lab technologists? I
am having a discussion with my general manager on who can prep pap smears. I
decided to take a quick poll to see what everyone else is doing. The lab is
in NYS so we fall under their guidelines, which I have checked. Just want as
much info as possible from as many sources as I can. Thanks in advance for
the responses.
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Supervisor
X-Cell Laboratories
716-250-9235 ext. 232
e-mail cpyse <@t> x-celllab.com
------------------------------
Message: 17
Date: Thu, 21 Apr 2011 10:10:02 -0400
From: Carol Bryant <cbrya <@t> lexclin.com>
Subject: [Histonet] processing times for skin specimens
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <50DA0C6B72976B4AB3A0FCA04CC73DBF05C77A9C7F <@t> EXCHANGESB>
Content-Type: text/plain; charset="us-ascii"
Hello everyone!
What is the shortest cycle you may be using for processing skin specimens? Can skin biopsies, shaves, and punches be processed well on a short run on the VIP 6? I need to find a successful protocol if possible to run skins on a 6 hour process, if possible.
Thank you in advance for any input.
Carol
Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com
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Message: 18
Date: Thu, 21 Apr 2011 10:00:22 -0400
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: [Histonet] IHC menu
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E02E1309B208F94C83B968E45781001A2350036E51 <@t> NCHEXMBX01.columbuschildrens.net>
Content-Type: text/plain; charset="us-ascii"
Would anyone be willing to share their IHC menu with us? I want to see how we compare with other top-class facilities. Obviously I am more interested in pediatric facilities, but we are constantly evaluating antibodies for research studies, and what may have applications in pediatric pathology from the adult world.
I'd be happy to send our menu on return
Thanks
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
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Message: 19
Date: Thu, 21 Apr 2011 07:23:36 -0700 (PDT)
From: Sheila Haas <micropathlabs <@t> yahoo.com>
Subject: Re: [Histonet] cyto prep
To: Cynthia Pyse <cpyse <@t> x-celllab.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <104166.87242.qm <@t> web161712.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
We have lab assistants running thin preps.
Our cytotechs screen and do HPV.
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
________________________________
From: Cynthia Pyse <cpyse <@t> x-celllab.com>
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Thu, April 21, 2011 9:51:35 AM
Subject: [Histonet] cyto prep
Good Morning Histonetters
For those of you who have pap smears in their facility's, who do you have
preparing the pap smears. Lab assistants, lab tech, or lab technologists? I
am having a discussion with my general manager on who can prep pap smears. I
decided to take a quick poll to see what everyone else is doing. The lab is
in NYS so we fall under their guidelines, which I have checked. Just want as
much info as possible from as many sources as I can. Thanks in advance for
the responses.
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Supervisor
X-Cell Laboratories
716-250-9235 ext. 232
e-mail cpyse <@t> x-celllab.com
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------------------------------
Message: 20
Date: Thu, 21 Apr 2011 10:25:03 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] Unsubscribe
To: Mollie Hannon <mollie <@t> phylogenyinc.com>, spanish_fly1981 <@t> yahoo.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BANLkTim1Lp4=WWS0EOcOOzzL5wsvPVm-qQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Jupiter's thunder!!!!
two people in a row? really?!!?!?
LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT YOU
SUBSCRIBED ON.
Both of you are officially banned from the internet. Forever. Don't use it
again.
And don't EVER join a mailing list. EVER.
Emily, who would love to unsubscribe you so I didn't have to get your
ridiculous emails about how you can't do it your damn self.
A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron
------------------------------
Message: 21
Date: Thu, 21 Apr 2011 10:31:13 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: RE: [Histonet] cyto prep
To: Cynthia Pyse <cpyse <@t> x-celllab.com>, 'Histonet'
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92AD9B20A6C38C4587A9FEBE3A30E164081F4BBA4D <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
We no longer do Gyn, but when we did we had lab assistants do the prep. Have lab asst for non-Gyn prep currently. J
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: Thursday, April 21, 2011 09:52
To: 'Histonet'
Subject: [Histonet] cyto prep
Good Morning Histonetters
For those of you who have pap smears in their facility's, who do you have preparing the pap smears. Lab assistants, lab tech, or lab technologists? I am having a discussion with my general manager on who can prep pap smears. I decided to take a quick poll to see what everyone else is doing. The lab is in NYS so we fall under their guidelines, which I have checked. Just want as much info as possible from as many sources as I can. Thanks in advance for the responses.
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology Supervisor
X-Cell Laboratories
716-250-9235 ext. 232
e-mail cpyse <@t> x-celllab.com
_______________________________________________
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Message: 22
Date: Thu, 21 Apr 2011 10:34:44 -0400
From: Merced Leiker <leiker <@t> buffalo.edu>
Subject: Re: [Histonet] Unsubscribe
To: Emily Sours <talulahgosh <@t> gmail.com>, Mollie Hannon
<mollie <@t> phylogenyinc.com>, spanish_fly1981 <@t> yahoo.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <91F17A8EA5A1AFDA3FBCAB34 <@t> CDYwxp1931.ad.med.buffalo.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed
**Listserv master: Some people are reporting problems with managing their
subscription as they have submitted their unsubscription multiple times in
the prescribed manner and are still getting Histonet emails. This was
reported to me by one such (failed) unsubscriber.**
--On Thursday, April 21, 2011 10:25 AM -0400 Emily Sours
<talulahgosh <@t> gmail.com> wrote:
> Jupiter's thunder!!!!
> two people in a row? really?!!?!?
> LOOK AT THE BOTTOM OF THE EMAIL. YOU UNSUBSCRIBE ON THE SAME PAGE THAT
> YOU SUBSCRIBED ON.
>
> Both of you are officially banned from the internet. Forever. Don't use
> it again.
> And don't EVER join a mailing list. EVER.
>
> Emily, who would love to unsubscribe you so I didn't have to get your
> ridiculous emails about how you can't do it your damn self.
>
> A great book should leave you with many experiences, and slightly
> exhausted. You should live several lives while reading it.
> -William Styron
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6118
Fx: (716) 829-2665
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
------------------------------
Message: 23
Date: Thu, 21 Apr 2011 11:18:23 -0500
From: Christi Cosby <christi.cosby <@t> gmail.com>
Subject: [Histonet] eosin bleeding on frozen sections
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BANLkTi=1QmkUzFnQ4S9ktpnNDfroeYob3w <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi,
In our lab we are having trouble with the eosin bleeding off the section
after coverslipping. We are performing a rapid H&E stain on frozen
sections. Any advice would be greatly appreciated!
------------------------------
Message: 24
Date: Thu, 21 Apr 2011 12:19:14 -0400
From: "Evans, Andria B" <aevans3 <@t> lghealth.org>
Subject: [Histonet] Neutralizing Formalin
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4182FDF23D7C9948BC41C4C082C3A54F021557B3AA65 <@t> MAIL-AG-CLUSTER.lha.org>
Content-Type: text/plain; charset="iso-8859-1"
Is anyone out there in Histoland Neutralizing their formalin before discarding it down the drain? We are looking into doing this because of our water authorities and we are finding that it is very costly. Has anyone found a cost efficient way to do this? I would need to know what supplier you are purchasing it from and the name of the product. Thank you in advance!!
Andria B Evans HTL(ASCP)CM
Lancaster General Hospital
555 North Duke Street
Lancaster, PA 17604
(717)544-5511 ext: 77329
This email was sent securely from the LGHealth Email Service
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------------------------------
Message: 25
Date: Thu, 21 Apr 2011 11:26:56 -0500
From: <sgoebel <@t> mirnarx.com>
Subject: RE: [Histonet] eosin bleeding on frozen sections
To: <christi.cosby <@t> gmail.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D957F2A7D21959488C492A2680F9920A223F7A <@t> svrexch.asuragen.us>
Content-Type: text/plain; charset="us-ascii"
This happened to me a couple weeks ago. You got water on the slide
after the eosin staining. Just run it back through alcohols, and rinse
for about five minutes. Change out the alcohols, and re-stain.
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Christi
Cosby
Sent: Thursday, April 21, 2011 11:18 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] eosin bleeding on frozen sections
Hi,
In our lab we are having trouble with the eosin bleeding off the section
after coverslipping. We are performing a rapid H&E stain on frozen
sections. Any advice would be greatly appreciated!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 26
Date: Thu, 21 Apr 2011 09:36:46 -0700 (PDT)
From: SHANE NELSON <nelsonrnch <@t> verizon.net>
Subject: [Histonet] (no subject)
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <193223.55791.qm <@t> web84301.mail.re1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi from Southern Cali,
We neutralize our formalin with a product called Neutralex. It comes in powder
form and after 30 mins we can dump it down the drain. We also keep
records for QC purposes. Product is purchased from a company called American
MasterTech. Hope this gives you a starting place.
THANK YOU,
PATTI RUBEN-NELSON H.T.(ASCP)
PNP LABORATORY CONSULTANT
SUPERVISOR/DESERT GASTROENTEROLOGY CONSULTANTS
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761
nelsonrnch <@t> verizon.net
------------------------------
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End of Histonet Digest, Vol 89, Issue 24
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