[Histonet] need ur help

Tue Dec 21 10:09:52 CST 2010

Thought I did. 

Thanks for making sure. 

Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

Tony Henwood <AnthonyH <@t> chw.edu.au> 
12/20/2010 04:14 PM

To
"'Kim.Donadio <@t> bhcpns.org'" <Kim.Donadio <@t> bhcpns.org>
cc
"'w_alkadhumi <@t> yahoo.com'" <w_alkadhumi <@t> yahoo.com>, 
"histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Subject
RE: [Histonet] need ur help

Kim,

Did you send this to Histonet & Wassan?

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

From: Kim.Donadio <@t> bhcpns.org [mailto:Kim.Donadio <@t> bhcpns.org] 
Sent: Tuesday, 21 December 2010 9:00 AM
To: Tony Henwood
Subject: RE: [Histonet] need ur help

Are the tiny biopsies freezing after you put them in paraffin? I have 
noticed that when you use freeze spray( in your case, maybe the coldness?) 
and the paraffin cracks it also cracks the tissue and you can see it 
microscopically. 

Just a thought. 

Kim Donadio 
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996 

Tony Henwood <AnthonyH <@t> chw.edu.au> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
12/20/2010 03:30 PM 

To
"'wassan alkadhumi'" <w_alkadhumi <@t> yahoo.com>, "
histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
cc

Subject
RE: [Histonet] need ur help

Check the fixative the biopsies are going into. Is it 10% neutral buffered 
formalin? 
Are they being fixed for long enough? Remember these are usually small, 
thin biopsies so they do not need extended processing so use the time to 
extend the fixation. You could try placing the biopsies in fixative in a 
warm oven (37-45oC) before loading onto the processor.
Are they being allowed to dry before being placed in fixative?

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of wassan 
alkadhumi
Sent: Tuesday, 21 December 2010 4:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] need ur help

Hi all
Hope u r all doing well, am an Iraqi histotech working in both 
histopathology and IHC department in Iraq. One part of what i do is IHC
(manual) i have no problem with, the other part is what i have problems 
with, Renals and Livers, we do regular H &E stain , PAS and Masson 
Tirchrome and all of them were text book quality until ten days ago, the 
problem is the Stains even the H&E are not Fine, its smugish. And the most 
important stain  for the histopathologist is the Trichrome which it should 
be fine and delacate and its not. I checked the procedures and stain 
preparations and i doing it the correctly , I made new solutions and did 
prolonged deparaffinization 10 min each step (3 xylenes,  2 100%ETOH,  one 
90%ETOH, one 70%ETOH) and there was no difference. I should mention that 
those problems happened with only Renal and Liver biopsies the H&E and PAS 
on other types of tissues are working just great. the one thing that 
changed before 10 days ago is the wether it became really cold and our lab 
temp. is not controlled.  Am going crazy please help me Thank u a lot 

Wassan
Histotech.
Shorsh hospital

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************************
This email and any files transmitted with it are confidential and intended 
solely for the use of the individual or entity to whom they are addressed. 
If you are not the intended recipient, please delete it and notify the 
sender.

Views expressed in this message and any attachments are those of the 
individual sender, and are not necessarily the views of The Children's 
Hospital at Westmead

This note also confirms that this email message has been virus scanned and 
although no computer viruses were detected, The Childrens Hospital at 
Westmead accepts no liability for any consequential damage resulting from 
email containing computer viruses.
*********************************************************************************

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

----------------------------------------- All electronic data 
transmissions originating from or sent to Baptist Health Care Corporation 
(BHC) are subject to monitoring. This message along with any attached 
data, are the confidential and proprietary communications of BHC and are 
intended to be received only by the individual or individuals to whom the 
message has been addressed. If the reader of this message is not the 
intended recipient, please take notice that any use, copying, printing, 
forwarding or distribution of this message, in any form, is strictly 
prohibited and may violate State or Federal Law. If you have received this 
transmission in error, please delete or destroy all copies of this 
message. For questions, contact the BHC Privacy Officer at (850) 434-4472. 
Rev.10/07.