[Histonet] need ur help

[Tony Henwood]

Kim,

Did you send this to Histonet & Wassan?

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

From: Kim.Donadio <@t> bhcpns.org [mailto:Kim.Donadio <@t> bhcpns.org]
Sent: Tuesday, 21 December 2010 9:00 AM
To: Tony Henwood
Subject: RE: [Histonet] need ur help


Are the tiny biopsies freezing after you put them in paraffin? I have noticed that when you use freeze spray( in your case, maybe the coldness?) and the paraffin cracks it also cracks the tissue and you can see it microscopically.

Just a thought.



Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996

Tony Henwood <AnthonyH <@t> chw.edu.au<mailto:AnthonyH <@t> chw.edu.au>>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu<mailto:histonet-bounces <@t> lists.utsouthwestern.edu>

12/20/2010 03:30 PM

To

"'wassan alkadhumi'" <w_alkadhumi <@t> yahoo.com<mailto:w_alkadhumi <@t> yahoo.com>>, "histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>" <histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>>

cc

Subject

RE: [Histonet] need ur help







Check the fixative the biopsies are going into. Is it 10% neutral buffered formalin?
Are they being fixed for long enough? Remember these are usually small, thin biopsies so they do not need extended processing so use the time to extend the fixation. You could try placing the biopsies in fixative in a warm oven (37-45oC) before loading onto the processor.
Are they being allowed to dry before being placed in fixative?

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of wassan alkadhumi
Sent: Tuesday, 21 December 2010 4:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] need ur help

Hi all
Hope u r all doing well, am an Iraqi histotech working in both histopathology and IHC department in Iraq. One part of what i do is IHC
(manual) i have no problem with, the other part is what i have problems with, Renals and Livers, we do regular H &E stain , PAS and Masson Tirchrome and all of them were text book quality until ten days ago, the problem is the Stains even the H&E are not Fine, its smugish. And the most important stain  for the histopathologist is the Trichrome which it should be fine and delacate and its not. I checked the procedures and stain preparations and i doing it the correctly , I made new solutions and did prolonged deparaffinization 10 min each step (3 xylenes,  2 100%ETOH,  one 90%ETOH, one 70%ETOH) and there was no difference. I should mention that those problems happened with only Renal and Liver biopsies the H&E and PAS on other types of tissues are working just great. the one thing that changed before 10 days ago is the wether it became really cold and our lab temp. is not controlled.  Am going crazy please help me Thank u a lot

Wassan
Histotech.
Shorsh hospital



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
*********************************************************************************

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu<mailto:Histonet <@t> lists.utsouthwestern.edu>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

----------------------------------------- All electronic data transmissions originating from or sent to Baptist Health Care Corporation (BHC) are subject to monitoring. This message along with any attached data, are the confidential and proprietary communications of BHC and are intended to be received only by the individual or individuals to whom the message has been addressed. If the reader of this message is not the intended recipient, please take notice that any use, copying, printing, forwarding or distribution of this message, in any form, is strictly prohibited and may violate State or Federal Law. If you have received this transmission in error, please delete or destroy all copies of this message. For questions, contact the BHC Privacy Officer at (850) 434-4472. Rev.10/07.