SPAM-LOW: RE: [Histonet] need ur help

[Patsy Ruegg]

Yea I would say make sure the samples are getting fixed long enough,
fixation is also temp dependent so if it is colder now you might have to fix
longer, try and fix your tissue, even biopsies for 24 hours if you can in
10% Neutral phosphate buffered formalin.

Regards,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tony Henwood
Sent: Monday, December 20, 2010 2:30 PM
To: 'wassan alkadhumi'; histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: RE: [Histonet] need ur help

Check the fixative the biopsies are going into. Is it 10% neutral buffered
formalin? 
Are they being fixed for long enough? Remember these are usually small, thin
biopsies so they do not need extended processing so use the time to extend
the fixation. You could try placing the biopsies in fixative in a warm oven
(37-45oC) before loading onto the processor.
Are they being allowed to dry before being placed in fixative?

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of wassan
alkadhumi
Sent: Tuesday, 21 December 2010 4:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] need ur help

Hi all
Hope u r all doing well, am an Iraqi histotech working in both
histopathology and IHC department in Iraq. One part of what i do is IHC
(manual) i have no problem with, the other part is what i have problems
with, Renals and Livers, we do regular H &E stain , PAS and Masson
Tirchrome and all of them were text book quality until ten days ago, the
problem is the Stains even the H&E are not Fine, its smugish. And the most
important stain  for the histopathologist is the Trichrome which it should
be fine and delacate and its not. I checked the procedures and stain
preparations and i doing it the correctly , I made new solutions
and did prolonged deparaffinization 10 min each step (3
xylenes,  2 100%ETOH,  one 90%ETOH, one 70%ETOH) and there was no
difference. I should mention that those problems happened with only Renal
and Liver biopsies the H&E and PAS on other types of tissues are working
just great. the one thing that changed before 10 days ago is the wether it
became really cold and our lab temp. is not controlled.  Am going crazy
please help me Thank u a lot 

Wassan
Histotech.
Shorsh hospital


      
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