[Histonet] need ur help

[Tony Henwood]

Check the fixative the biopsies are going into. Is it 10% neutral buffered formalin? 
Are they being fixed for long enough? Remember these are usually small, thin biopsies so they do not need extended processing so use the time to extend the fixation. You could try placing the biopsies in fixative in a warm oven (37-45oC) before loading onto the processor.
Are they being allowed to dry before being placed in fixative?

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of wassan alkadhumi
Sent: Tuesday, 21 December 2010 4:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] need ur help

Hi all
Hope u r all doing well, am an Iraqi histotech working in both histopathology and IHC department in Iraq. One part of what i do is IHC
(manual) i have no problem with, the other part is what i have problems with, Renals and Livers, we do regular H &E stain , PAS and Masson Tirchrome and all of them were text book quality until ten days ago, the problem is the Stains even the H&E are not Fine, its smugish. And the most important stain  for the histopathologist is the Trichrome which it should be fine and delacate and its not. I checked the procedures and stain preparations and i doing it the correctly , I made new solutions and did prolonged deparaffinization 10 min each step (3 xylenes,  2 100%ETOH,  one 90%ETOH, one 70%ETOH) and there was no difference. I should mention that those problems happened with only Renal and Liver biopsies the H&E and PAS on other types of tissues are working just great. the one thing that changed before 10 days ago is the wether it became really cold and our lab temp. is not controlled.  Am going crazy please help me Thank u a lot 

Wassan
Histotech.
Shorsh hospital


      
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