[Histonet] GFAP IHC

[Amy Porter]

If I remember correctly Dako's GFAP is a bovine immunogen (bovine spinal 
cord) - that is probably what is causing the problem.  You may want to look 
for GFAP where the cow is not the immunogen / try running another brain from 
a different species and see if you get the same problem.

Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg.  Rm #2133
East Lansing, MI  48824-3320
Phone:  (517) 884-5026
Fax:  (517) 432-1368
Email:  portera <@t> msu.edu
Web:  www.humanpathology.msu.edu
----- Original Message ----- 
From: "Nancy Herman" <Nancy.Herman <@t> inspection.gc.ca>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, October 28, 2009 4:19 PM
Subject: [Histonet] GFAP IHC


> We are using DAKO's ready-to-use GFAP antibody on our bovine tissue and 
> using Envision+ HRP Rabbit detection system.  When using this antibody we 
> see a brown color on the slide itself.  The staining was intense and quite 
> uneven.  We have diluted the anitbody to 1:10 but there is still the brown 
> slide.  The staining is not quite as uneven but appears more washed out. 
> We don't see this with any of our other antibodies we are using and our no 
> primary controls for GFAP are clean.  Any advice or explanations for the 
> slides picking up the brown color or improving GFAP IHC on bovine tissue?
> Thanks
>
> Nancy Herman
> CFIA Lethbridge Laboratory
> Lethbridge, Alberta


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