[Histonet] Masson's tri-chrome specificity

Jennifer Anderson janderson <@t> halozyme.com
Fri Oct 23 12:51:45 CDT 2009


Good morning Histonet.

Thanks a lot to so many people who helped me with my Leica XL question - your wealth of knowledge never ceases to impress me.

I have a different question now about Masson's tri-chrome and it's specificity on isolated rabbit tendon.  There is some muscle still attached to the ends of the tendon.  We get both Beibrich Scarlet and Aniline Blue staining, at different intensities, within each collagen sample (even control).  How can I explain this?  Would there be a possibility to omit the Beibrich and stain with eosin and Aniline blue?

Thanks so much!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333
janderson <@t> halozyme.com<mailto:janderson <@t> halozyme.com>



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