[Histonet] CD 31 in rats

[Melanie Black]

Hi Patsy

I have tried RECA-1 and not had great success. The Fitzgerald one is  
the best and we have researched this plenty.

One option for PFA fixed rat tissue is the Abcam VWF ( ab6994). I use  
it 1:400 overnight incubation of primary with 10 minutes proteinase K  
before hand, followed by Donkey anti-Rabbit Cy 3 (fluorescent stain).  
Works well.

Melanie.


Melanie Black
082 469 3352

Cardiovascular Research Unit
3rd Floor; Chris Barnard Building
Medical School;
Observatory. 7925.
University of Cape Town.
South Africa.