[Histonet] Re: Histonet Digest, Vol 71, Issue 18

[Nick and Amanda]

Hi can anyone please tell me who supplies Pgfr antibody?

Manda in Wellington






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Sent: Monday, October 19, 2009 6:03 AM
Subject: Histonet Digest, Vol 71, Issue 18


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> Today's Topics:
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>   1. Re: Processing Whole prostate (Robert Richmond)
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> Message: 1
> Date: Sat, 17 Oct 2009 16:56:37 -0400
> From: Robert Richmond <rsrichmond <@t> gmail.com>
> Subject: [Histonet] Re: Processing Whole prostate
> To: histonet <@t> lists.utsouthwestern.edu
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> 
> I don't have any experience with whole-mount sections of radical
> prostatectomy specimens, which obviously require slides larger than
> the standard 3 by 1 inch slide.
> 
> I'm not entirely current here, but a few years ago major prostate
> pathologists such as Jonathan Epstein (at Johns Hopkins) were telling
> us that while whole-mount sections were necessary for in research
> settings such as his own, that they were distinctly not required as
> standard of care in community hospitals.
> 
> I prefer to let a whole radical prostatectomy specimen sit overnight
> in neutral buffered formalin (without removing the urethral catheter
> if there is one), then cut it, then further fix the sections
> overnight. Fixation isn't wonderful, but remember that most of what I
> want to see is near the exterior surfaces of the specimen. I flick out
> any of those little black prostatic calculi I may see.
> 
> The right and left halves of the prostate should be marked with
> contrasting colors of ink, either before or after fixation, but before
> cutting. Learning to orient the specimen is a non-trivial task.
> 
> I wouldn't invest much money in setting up whole-mount sections. I
> expect the numbers of these specimens to decrease, as the futility of
> treating early prostate cancer is more widely appreciated.
> 
> Bob Richmond
> Samurai Pathologist
> Knoxville TN
> 
> 
> 
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> End of Histonet Digest, Vol 71, Issue 18
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