[Histonet] Labvision immunostainers skip slides

[Hoekert, W.E.J.]

Thank you all for your replies, they are of great use to me. I now understand how the skipped staining can occur. I have told our support guy to look at the reagent sensor system, and he will do so today. He told me that he has already replaced them a while ago, and that the new ones are relatively stable. However, he also told me that a few weeks ago, they changed the settings of the probe, so that the probe is now descending to the bottom of the vial when it is sucking up the reagents. So actually, I still don't understand how we can get the skipped staining. I am afraid that this problem will not be fixed very easy, if it can be fixed at all. I understand that Labvision is working on this problem and that in october next year they hope to have a solution.
 
We have decided to deparaffinize with xylene since it seems that the PT modules are not effective enough in doing so. We will keep a closer eye on them as some of you have pointed out that they can be a cause of problems. We also have irregular staining, but this occurs also with slides that have not been treated in the PT modules.
 
PS: we use Tris Hcl buffer (10 times concentrated) from Klinipath, which contains tween already. And yes, we do get good support, but still the problems are not solved. 
 
Thanks again, 

Willem Hoekert
Pathology Lab, OLVG 
Amsterdam
The Netherlands
 


 
________________________________

Van: histonet-bounces <@t> lists.utsouthwestern.edu namens Hoekert, W.E.J.
Verzonden: vr 9-10-2009 13:19
Aan: histonet <@t> lists.utsouthwestern.edu
Onderwerp: [Histonet] Labvision immunostainers skip slides



Dear Histonetters,


A few months ago, we bought 2 Labvision Autostainers 720 from Thermo (immunostainers). We have some bad experiences with them.

They seem to skip some slides every now and than (it happens maybe once every 3-4 weeks, we stain about 150 - 200 slides a day). If so, our positive controls are virtually completely negative, and the patient slides are negative as well. If this happens, we see empty 'holes' in the nuclei. Click here to see an image: http://www.immunoportal.com/modules.php?name=gallery2&g2_itemId=14523&g2_imageViewsIndex=1

All the other antibodies from the same run work fine, and if we repeat the failed cases (without changing anything) they turn out fine.

Does anybody recognize this problem or knows what is going on?

We dewax in PT modules (the problem occurs both with citraclere and with PT Module Buffer 1 from immunologic).

I hope to hear from you.

Willem Hoekert







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Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. 
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