[Histonet] Labvision immunostainers skip slides

[Hoekert, W.E.J.]

Dear Histonetters,


A few months ago, we bought 2 Labvision Autostainers 720 from Thermo (immunostainers). We have some bad experiences with them. 

They seem to skip some slides every now and than (it happens maybe once every 3-4 weeks, we stain about 150 - 200 slides a day). If so, our positive controls are virtually completely negative, and the patient slides are negative as well. If this happens, we see empty 'holes' in the nuclei. Click here to see an image: http://www.immunoportal.com/modules.php?name=gallery2&g2_itemId=14523&g2_imageViewsIndex=1

All the other antibodies from the same run work fine, and if we repeat the failed cases (without changing anything) they turn out fine. 

Does anybody recognize this problem or knows what is going on? 

We dewax in PT modules (the problem occurs both with citraclere and with PT Module Buffer 1 from immunologic).

I hope to hear from you.

Willem Hoekert

 


 


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